Type 1 NSPCs express nestin, ApoE, and GFAP, while mature granule cells make connections with astrocytes expressing ApoE and GFAP. A–D, Representative confocal images of the dentate gyrus illustrate nestin-expressing (red), ApoE-expressing (green), and GFAP... Type 1 NSPCs express nestin, ApoE, and GFAP, while mature granule cells make connections with astrocytes expressing ApoE and GFAP. A–D, Representative confocal images of the dentate gyrus illustrate nestin-expressing (red), ApoE-expressing (green), and GFAP-expressing (blue) cells, along with a merged image of all channels. E–H, High-power representative confocal images taken from the region in the inset in D illustrate nestin-expressing (red), ApoE-expressing (green), and GFAP-expressing (blue) cells, along with a merged image of all channels. White arrows indicate nestin-ApoE-GFAP triple-positive cells, representing type 1 NSPCs. I–L, Representative confocal images of the dentate gyrus illustrate tdTomato-expressing (red), ApoE-expressing (green), and GFAP-expressing (blue) cells, along with a merged image of all channels. M–P, High-power representative confocal images taken from the region in the inset in L illustrate tdTomato-expressing (red), ApoE-expressing (green), and GFAP-expressing (blue) cells, along with a merged image of all channels. White arrows indicate GFAP and ApoE coexpressing astrocytes. GL, Granule layer; HIL, hilus. Scale bars: D, 100 µm; H, 75 µm; L, 100 µm; P, 25 µm. Yacine Tensaouti et al. eNeuro 2018;5:ENEURO.0155-18.2018 ©2018 by Society for Neuroscience