1. DFP-induced astrogliosis is reduced by urethane.
DFP-induced astrogliosis is reduced by urethane. Representative images (taken with a 10× objective lens) showing positive GFAP immunostaining (A) as an astrocyte marker and Iba1 immunofluorescence staining (B) as a microglial marker in the hippocampal CA3 region (DAB with a Nissl counterstain). Four days after DFP-induced SE, astrogliosis, and microgliosis were obvious in the sections obtained from rats as defined by the increased number of positively labeled cells in rats that experienced uninterrupted SE and rats administered diazepam 1 h after SE onset but not as much in sections taken from rats injected with urethane 1 h following SE onset or non-seizure controls. Scale bar, 100 μm. C, Induction of GFAP, CD11B and Iba1 mRNA in the forebrain 4 d following DFP SE in rats that experienced uninterrupted SE (n = 7), rats administered diazepam 1 h after SE onset-treated (n = 9 rats) and urethane-treated (1 h SE) rats (n = 13 rats; ***p < 0.001, one-way ANOVA with post hoc Bonferroni).
Asheebo Rojas et al. eNeuro 2018;5:ENEURO
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