Comparison of the variable regions of (A) pHNZY32, pHNZY118, and pHNAH24; (B) pHNMCC14; (C) pHNFKU92; (D) pE80; (E) pECB11; (F) p42-2; and (G) pSLK172-2.

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Comparison of the variable regions of (A) pHNZY32, pHNZY118, and pHNAH24; (B) pHNMCC14; (C) pHNFKU92; (D) pE80; (E) pECB11; (F) p42-2; and (G) pSLK172-2. Comparison of the variable regions of (A) pHNZY32, pHNZY118, and pHNAH24; (B) pHNMCC14; (C) pHNFKU92; (D) pE80; (E) pECB11; (F) p42-2; and (G) pSLK172-2. The extents and directions of antibiotic resistance in antibiotic resistance genes (thick arrows) and in other genes are indicated. Genes in pE80 (D) could not be annotated because of nucleotide changes, and deletions are indicated by red arrows. ISs are shown as boxes labeled with the IS name. Labeled vertical arrows with IS boxes indicate the insertion sites of IS elements. Direct repeats are indicated by arrows and sequence. Tall bars represent the 38-bp TIR of transposons. The backbone is indicated by dotted lines. Arrows labeled with "HR" and dotted lines indicate where homologous recombination could explain differences between structures. Target sites of IS1294 are labeled with black arrows. Adjacent regions are connected with dotted lines. Sequences referred to in the diagram are from the GenBank accession numbers listed in Table 1. Jing Wang et al. mSphere 2018; doi:10.1128/mSphere.00137-18