1. Accumulation of transcripts from MULE‐F19G14–CyP40 and transposons in mom1 and ddm1.
Accumulation of transcripts from MULE‐F19G14–CyP40 and transposons in mom1 and ddm1. (A) RNA blot analysis of MULE‐F19G14/CyP40 transcripts. Total RNAs extracted from Col‐0 (wild type), ddm1‐2 and mom1‐2 were probed with CyP40 and MULE‐F19G14 probes. Positions of the size markers are shown on the right in kilobases (kb). (B) A chromosomal region containing CyP40 and MULE‐F19G14. Filled and open boxes represent exons and introns, respectively. Grey boxes represent terminal inverted repeats of MULE‐F19G14. The direction and position of annotated genes are indicated by arrows. A deduced structure of the 7‐kb transcript is shown by bars connected with dashed lines corresponding to introns. The positions of probes for RNA blots and ChIP are indicated at the bottom. The main transcription start site observed in mom1 and ddm1 is indicated by a hooked arrow. (C) Accumulation of the transcripts of AtMu1. Reverse transcription–PCR (RT–PCR) was carried out using RNA samples for Col‐0 (wild type, wt), ddm1‐2, and mom1‐2 with (+) or without (−) RT. (D) Accumulation of transcripts of Tar17. RNA blot analysis was carried out using total RNA of Col‐0 (wild type (wt)), ddm1‐2 and mom1‐2. ChIP, chromatin immunoprecipitation; CyP40, Cyclophilin 40; ddm1, decrease in DNA methylation 1; mom1, Morpheus' molecule 1; MULE, Mutator‐like element.
Yoshiki Habu et al. EMBO Rep. 2006;7:
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