How to Test an Assertion Raw material Cys Cys Asp Glu Glu Glu Glu Ser Gly Gly Gly Thr Ala Ala Tyr Tyr Val Val Leu Leu Leu Leu Phe Phe Phe Pro His His Lys Arg

Build and Test an Assertion Example 1 Raw material A. Gly-Val Table 6, Line 8 B. Gly-(Ala,Lys,Val) Table 9, Line 6 C. Gly-Val-(Ala,Lys) Assertions A+B ??? Cys Cys Asp Glu Glu Glu Glu Ser A. Gly Val Gly Gly Gly Thr Ala Ala Ala B. Gly Val Tyr Tyr Lys Val Val Leu Leu Leu Leu Ala C. Gly Val Phe Phe Phe Lys Pro Does C explain A & B? His His Lys Arg

Build and Test an Assertion Example 1 Raw material A. Gly-Val Table 6, Line 8 B. Gly-(Ala,Lys,Val) Table 9, Line 6 C. Gly-Val-(Ala,Lys) Assertions A+B+C ??? Cys Cys Asp Glu Glu Glu Glu Ser A. Gly Val Gly Gly Thr Ala Ala B. Gly Val Tyr Tyr Lys Val Leu Leu Leu Leu Ala C. Gly Gly Val Val Phe Phe Phe Lys Pro Does C explain A & B? His His Yes… is it the only way? Arg

Build and Test an Assertion Example 1 Raw material A. Gly-Val Table 6, Line 8 B. Gly-(Ala,Lys,Val) Table 9, Line 6 C. Gly-Val-(Ala,Lys) Assertions A+B+C ??? Cys Cys Asp Glu Glu Glu Glu Ser A. Gly Val Gly Thr Ala Ala B. Gly Val Tyr Tyr Lys Leu Leu Leu Leu C. Gly Gly Val Val Gly Ala Lys Val Phe Phe Phe Pro Does C explain A & B? His His Yes… is it the only way? Arg No… so the assertion’s wrong

Build and Test an Assertion Example 2 Raw material A. Thr-Pro Table 6, Line 8 B. Thr-(Ala,Lys,Pro) Table 9, Line 6 C. Thr-Pro-(Ala,Lys) Assertions A+B+C ??? Cys Cys Asp Glu Glu Glu Glu Ser A. Thr Pro Gly Gly Gly Thr Ala Ala Ala B. Thr Pro Tyr Tyr Lys Val Val Leu Leu Leu Leu Ala C. Thr Pro Phe Phe Phe Lys Pro His His Lys Arg

Build and Test an Assertion Try your own Raw material Cys Cys Asp Glu Glu Glu Glu Ser Gly Gly Gly Thr Ala Ala Tyr Tyr Val Val Leu Leu Leu Leu Phe Phe Phe Pro His His Lys Arg