Fig. 5. Remnants of PS+ platelets induce neutrophil macroaggregation.

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Fig. 5. Remnants of PS+ platelets induce neutrophil macroaggregation. Remnants of PS+ platelets induce neutrophil macroaggregation. (A) Representative differential interference contrast (DIC) images depicting spread human platelets pre-Thr/CRP stimulation (Thr/CRP 0′), remnant platelets post-Thr/CRP stimulation (Thr/CRP 9.3′) after adhesion to fibrinogen, and shed microparticles from Thr/CRP-stimulated platelets in suspension. Scale bar, 1 μm. (B and C) Representative phase contrast images depicting neutrophil macroaggregate formation on remnant PS+ platelets (B) or PS− platelets (C) at the indicated shear rates (n = 3). (D) Quantification of the percentage of adherent neutrophils aggregated (left) and size of neutrophil aggregates formed (right) after neutrophil perfusion over remnant PS+ platelets, relative to microparticle coperfusion (1× or 10 to 100× MPs) over PS− platelets. Error bars represent means ± SEM (n = 3). ****P < 0.001. (E) Representative confocal images depicting the extent of incorporation of large remnant PS+ platelet membrane fragments within aggregating neutrophils on remnant PS+ platelets or after microparticle coperfusion (1× MP) over PS− platelets (n = 3 to 5). Scale bars, 10 μm. Yuping Yuan et al., Sci Transl Med 2017;9:eaam5861 Published by AAAS