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Fig. 1. BCAS1 expression identifies newly generated oligodendrocytes.

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1 Fig. 1. BCAS1 expression identifies newly generated oligodendrocytes.
BCAS1 expression identifies newly generated oligodendrocytes. (A) Confocal images of breast carcinoma amplified sequence 1 (BCAS1) (pink) and oligodendrocyte transcription factor 2 (OLIG2) (white) in the corpus callosum (CC) of postnatal day 40 (P40) mice. Scale bars, 5 μm. (B) Bar graph shows the percentage of BCAS1+ cells expressing sex-determining region Y box 10 protein (SOX10), OLIG1, or OLIG2. (C) Bar graph shows the percentage of BCAS1+ cells expressing neural/glial antigen 2 (NG2), CC1, or myelin-associated glycoprotein (MAG) in the corpus callosum of P40 mice. (D) Left (experimental plan): NG2-CreERT2:R26R-tdTomato-mEGFP mice were used to induce the expression of mEGFP by tamoxifen (TMX) at P30 as illustrated. Right: Quantification of the percentage of double-positive [GFP+ (green fluorescent protein–positive) and BCAS1+] cells over time. One-way analysis of variance (ANOVA), followed by Bonferroni’s post hoc test, *P < 0.05; n = 3, three sections per animal. Quantitative data are means ± SEM. Maryam K. Fard et al., Sci Transl Med 2017;9:eaam7816 Published by AAAS


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