1. Fig. 7 MRSA virulence proteins cause LMC death and diminished CLV function.
MRSA virulence proteins cause LMC death and diminished CLV function. (A) mLMC viability analysis after exposure for 24 hours to control medium (TSB), agr mutant–conditioned medium, or MRSA-conditioned medium; n = 3 independent experiments. (B) LDH release from LMCs incubated with conditioned medium from WT or agr mutant MRSA; n = 3 independent experiments. (C) Computer-automated quantification of % αSMA-positive area of PLV in uninfected animals and animals 30 days after agr mutant MRSA infection; n = 3 to 4 mice for each group. (D) Representative traces from lymphatic vessel wall measurements show lymphatic diameter and contraction over time in uninfected mice and mice infected with WT or agr mutant MRSA for 30 days. (E) Ejection fraction shows the strength of lymphatic vessel contraction in uninfected mice and mice infected with WT or agr mutant MRSA for 30 days; n = 8 to 17. (F) Frequency indicates the cpm of lymphatic vessel contractions in uninfected mice and mice infected with WT or agr mutant MRSA for 30 days; n = 8 to 15. For (E) and (F), statistical analysis was performed by one-way ANOVA comparison with Fisher’s least significance difference post hoc analysis. Error bars show SEM. *P < 0.05.
Dennis Jones et al., Sci Transl Med 2018;10:eaam7964
Published by AAAS