1. Fig. 7. Inhibitory mechanisms of C12G6.
Inhibitory mechanisms of C12G6. (A) MDCK cells were inoculated with B/Florida/4/2006 (FL/2006) or B/Brisbane/60/2008 (BR/2008) viruses preincubated with C12G6 or polyclonal rabbit sera, as indicated. The expression of influenza B nucleoprotein in MDCK cell monolayers 16 to 18 hours after inoculation was detected by immunofluorescence. Green, infected cells positive for NP protein; blue, 4′,6-diamidino-2-phenylindole staining. (B) Immunoblots of influenza B detected in the lysates and supernatants of MDCK cells infected with B/Florida/4/2006 or B/Brisbane/60/2008 and subsequently incubated with different concentrations of C12G6, as indicated. Influenza B was detected using rabbit sera against B/Florida/4/2006 and B/Brisbane/60/2008, respectively. (C) C12G6 protects HAs of B/Florida/4/2006 and B/Brisbane/60/2008 from the pH-induced protease sensitivity associated with membrane fusion. (D to G) ADCC (D and E) or CDC (F and G) activities of the indicated antibodies against B/Florida/4/2006 virus– and B/Brisbane/60/2008 virus–infected MDCK cells, respectively. The bars represent the mean ± SEM. *P < 0.05, compared to the control IgG group.
Chenguang Shen et al., Sci Transl Med 2017;9:eaam5752
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