ERYTHROBLASTOSIS FETALIS PREVENTION OF THE DISEASE A potentially life threatening blood disorder in a foetus or a newborn, arising due to incompatibility with mother’s blood.‏ Title of the concept SUPRIYA SARASWATI

Master layout or diagram INSTRUCTIONS SLIDE 1 Master layout or diagram Make a schematic diagram of the concept Explain the animator about the beginning and ending of the process. Draw image big enough for explaining. In above image, identify and label different components of the process/phenomenon. (These are like characters in a film)‏ Illustrate the basic flow of action by using arrows. Use BOLD lines in the diagram, (minimum 2pts.)‏ In the slide after that, provide the definitions of ALL the labels used in the diagram 2 3 4 5

MASTER LAYOUT Part Four: PREVENTION OF EF Rh- mother receiving anti Rh antibodies injection

Definitions of the components: 1 1. Anti Rh antibodies: These are antibodies to the Rh factor that are administered into the mother during anytime there is a chance of mixing of the mother’s blood with that of the fetal blood. This could be anytime during pregnancy, labour or birth. 2 3 4 5

1 Explain the process 2 In this step, use an example to explain the concept. It can be an analogy, a scenario, or an action which explains this concept/process/topic Try to use examples from day-to-day life to make it more clear You have to describe what steps the animator should take to make your concept come alive as a series of moving images. Keep the examples simple to understand, and also to illustrate/animate. 3 4 5

Analogy / Scenario / Action 1 Analogy / Scenario / Action 2 The immune system of the body is well developed to fight infections and keep the body healthy. Any foreign substance (antigen) entering the body makes the immune system produce molecules called antibodies that destroy the antigen. Blood transfusions are procedures of transferring blood or blood based products from one circulatory system into another. For this the blood of the donor should be compatible with that of the recipient’s blood, which means the blood types should match. Or else any component not matching the recipient’s blood type will lead to production of antibodies in his body against the donor’s blood cells. These antibodies will attack the newly received blood and kill the blood cells. Erythroblastosis fetalis is a diseased condition arising from incompatibility of fetal blood with that of the maternal blood. It usually happens in Rh- women carrying Rh+ fetus. Maternal antibodies attack the fetal blood cells and destroy them. 3 4 5

Stepwise description of process 1 The goal of the IDD is to provide instructions to an animator who is not a expert. You have to describe what steps the animator should take to make your concept come alive as a moving visualization. Use one slide per step. This will ensure clarity of the explanation. Add a image of the step in the box, and the details in the table below the box. You can use any images for reference, but mention about it's copyright status The animator will have to re-draw / re-create the drawings Add more slides as per the requirement of the animation 2 3 4 5

3 Step 23: 1 2 4 5 Action Description of the action Audio Narration IgG anti Rh antibodies are administered to the mother, against the Rh antigen after any chance event that could lead to mixing of the fetal blood with that of mother’s. Show Rh- woman being injected Show Rh- woman being injected with IgG anti Rh antibodies. Show IgG anti Rh antibody molecules entering her circulation. 5

3 Step 24: 1 2 4 5 Action Description of the action Audio Narration Antibodies administered to the mother facilitate the clearance of fetal RBCs from mother’s circulation before memory cell production can take place and pose threat to the fetus. Show binding of IgG anti Rh antibodies to fetal RBCs. Show that the administered antibodies bind to any fetal RBCs present in the mother’s blood. 5

3 Step 25: 1 2 4 5 RhoGAM MICRhoGAM Gamulin Rh Bay Rho-D Action Description of the action Audio Narration Show different vials labeled with different names of commercially available IgG anti Rh antibodies, as shown in the figure. IgG anti Rh antibodies are used for preventive measures in cases of erythroblastosis fetalis. These are available commercially under various brand names. Show brand names of IgG anti Rh antibodies 5

Animation design 1 Please see the design template provided in the next slide. This is a sample template, and you are free to change as per your design requirements. Try and recreate the sections/subsections as shown in the template. 2 3 4 5

Interactivity and Boundary limits 1 Interactivity and Boundary limits In this section, you will add the ‘Interactivity’ options to the animation. Use the template in the next slide to give the details. Insert the image of the step/s (explained earlier in the Section 3) in the box, and provide the details in the table below. The details of Interactivity could be: Types: Drop down, Slider bar, Data inputs etc. Options: Select one, Multiple selections etc Boundary Limits: Values of the parameters, which won’t show results after a particular point Results: Explain the effect of the interaction in this column Add more slides if necessary 2 3 4 5

Interactivity option 1:Step No: Which of these should be injected into Rh- mother carrying Rh+ fetus for prevention of erythroblastosis fetalis? Dexamethasone 2 BCG RhoGAM 3 C A B 4 Interacativity Type Options Boundary/limits Results The correct answer box turns green on clicking. The incorrect answer box turns red on clicking. Box C is the correct answer. Boxes A and B have incorrect answers. Choosing the correct option box 5

Links for further reading Reference websites: www.nlm.nih.gov http://en.wikipedia.org www.marchofdimes.com www.mercksource.com Books: Textbook of Immunology, Kuby Textbook of Medical Physiology, Guyton Research papers: