HC70AL Research Presentation Eric Newton

First gene AT3G47640 Wild Type: 1,409 Mutant: 730

Second gene AT3G61310 Wild Type: 621 Mutant: 609

First gene plants genotype Plant 1: Heterozygous Plant 2: WT Plant 3: WT Plant 4: Mutant Plant 5: WT Plant 6: WT Plant 7: WT Plant 8: -undetermined- Plant 9: Mutant Plant 10: WT Plant 11: Mutant Plant 12: -undetermined-

Gene 1 mutant plant phenotype Why no phenotype for 2nd gene?

Second gene plants genotype

Salk vs. my results Gene anatomy according to Salk online database Gene anatomy according to my lab results

When and where are the genes expressed? Second Gene First Gene

Promoter region 1 What were the sequence results? 1-kb Ladder Clone #1

Promoter region gene 2 What were the sequence results?

Conclusion Gene 1 -found mutant -no visible phenotype -Promoter cloning obsolete Gene 2 -no found mutant -phenotype N/A -Promoter cloning has mutations