1. HC70AL Oral Presentation
Roman Groisberg
Spring 2004

2. YES! Knockout in Salk line 006218 What is the structure of AT3G09735?
Does a Knockout exist for AT3G09735?
Knockout in Salk line
Madison DNA pool 141
Madison Superpool 16

3. Where is AT3G09735 expressed in the Arabidopsis thaliana?
Expressed at higher
Levels in maturation stages!
Where is the ortholog for AT3G09735 gene expressed in Scarlet Runner Bean?
Expressed in all organs
Except flowers.

4. What protein does AT3G09735 encode for?
S1Fa DNA Binding protein
What is the predicted structure of the protein?
Coil-Helix-Coil-Helix-Coil
Protein kinase C phosphorylation site
N-myristoylation site
What is the function of the protein?
Gene repressor

5. What are the genotyping results?
Salk plants 1-9:
Fw/Rv primer
+Tubulin
T-DNA/RV
+Tubulin
Conclusion:
No T-DNA insertions in plants 1-18

6. What are the phenotyping results?
Wild type:
Salk line :
Trichome
Silique
Flower
Conclusion: No Difference

7. Cannot be determined without further research
Is AT3G09735 important for seed development?
Cannot be determined without further research
What is next?
Genotype more Salk Plants to find:
homozygous T-DNA
heterozygous T-DNA
Genotype plants from other
facilities that also have a
knockout for at3g09735
Cross plants with knockouts in other S1Fa
family genes and see if seeds develop with
multiple S1Fa genes knocked out
Crystalize protein to determine actual structure of S1Fa