1. Are At1g08810 and At3g50060 Important to Arabidopsis Seed Development?
Jonathan Russell
June 9, 2005
HC70AL

2. What are the Structures of My Genes?
At1g08810
At3g50060

3. What Information is Important?
At1g08810
At3g50060
Protein Type
MYB 60
MYB 77
Size (bp)
1,331
1,135
Size (aa)
280
301
# of Exons 3 1
# of Introns 2
Most Active
Mature Green Stage Seed
Post-Mature Green Stage Seed

4. When/Where are My Genes Active?
At1g08810
At3g50060

5. What Activity Did Experiments Show?
At1g08810
Expected Size of RT-PCR Product
At1g08810
295 bp
At3g50060
576 bp
At3g50060

6. What Were My 1st Line’s Genotypes?
Expected Size of PCR Product
At1g08810
Wild Type
1,131 bp
T-DNA
663 bp

7. What Were My 1st Line’s Genotypes?
Expected Size of PCR Product
At1g08810
Wild Type
1,131 bp
T-DNA
663 bp

8. What Were My 2nd Line’s Genotypes?
Expected Size of PCR Product
At3g50060
Wild Type
1,496 bp
T-DNA
1,258 bp

9. Genotype Summary At1g08810 At3g50060 WT / WT 16 WT/ Mut 3 Mut / Mut 2
WT/ Mut 3
Mut / Mut 2 22

10. Did the Gene Maps Need Revision?
At1g08810: YES
At3g50060: YES

11. Do the Mutants Show a Phenotype?
Wild Type
At1g08810: NO
At3g50060: NO

12. Does Nomarski Reveal a Phenotype?
Wild Type
At3g50060: NO
At1g08810: NO

13. How is Promoter Cloning Useful?
Amplification of Promoter Region (Gene 1)
EcoR1 Digestion of Plasmid Vector
(Gene 2)
Sequencing of
Promoter Region
At1g08810
At3g50060
SP6 T7
Matches
629
619
463
785
Ignored 8 86
Mismatch 1 4
Expected Size of Promoter Region
At1g08810
1,480 bp
At3g50060
3,153 bp

14. What Conclusions Can be Drawn?
My experiments did not reveal a mutant phenotype for either At1g08810 or At3g50060
Possible Explanations…
These genes are not vital for development
These genes are vital for development, but similar genes exist that can take over their functions
A mutant phenotype exists, but was not visible in my specific experiments