Poly-GR mainly co-localizes with nucleolar ribosomes.

Slides:

Advertisements

Similar presentations

Fig. 2. Expression of Ninj1 in cortices of ischemic hemispheres of rat brains at 1 day post-MCAO. Coronal brain sections were prepared at 1 day post-MCAO.

Advertisements

Poly-GR and poly-PR interact with overlapping proteins in primary neurons and in HEK293 cells. Poly-GR and poly-PR interact with overlapping proteins in.

Nuclear Factor of Activated T Cells 5 Deficiency Increases the Severity of Neuronal Cell Death in Ischemic Injury Neurosignals 2012;20:237–251 - DOI: /

FigS3 IHC analysis of Drosha and DGCR8 protein distribution in the hippocampus, frontal cortex and cerebellum of control cases. In the hippocampus both.

STAU1/2 and YBX1 recruit poly-GR/PR into large cytoplasmic granules.

Exposure to stress and SGs formation.

NPM1 drives poly-GR into the nucleolus of primary neurons.

Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients. Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients.

Aberrant wound architecture and proliferation in Rev3l-deficient skin.

Cytoplasmic poly-GR/PR inclusions resemble stress granules in vitro.

Characterization of DPR proteins in primary rat neurons and HEK293 cells. Characterization of DPR proteins in primary rat neurons and HEK293 cells. GFP,

Increased astrocyte proliferation is paralleled with reduced infiltration of immune cells in mice with inducible SMOM2 expression in astrocytes at 5 dpi.

Poly-GR and poly-PR interact with similar low-complexity proteins in neurons. Poly-GR and poly-PR interact with similar low-complexity proteins in neurons.

LGN is essential for Plk1-dependent cortical NuMA enrichment during metaphase, but not in anaphase. LGN is essential for Plk1-dependent cortical NuMA enrichment.

Figure 1 Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient.

Topisirovic et al., (2003) EMBO J,22:

Poly-PR inhibits translation in neurons.

Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. GFP,

Candidates with a PVM localization.

Fig. 7 CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα. CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα.

Marker identification and quantification by StrataQuest, and confocal analysis of EV-containing tissue sections. Marker identification and quantification.

Juxtapositioning of ARF5 with IRES-luc mRNA.

Subcellular localization of UUKV, HRTV, and SFTSV NSs

Juxtaposition of GFP-Rab1b with IRES-containing RNA.

Bexarotene is neuroprotective in mouse and human HD neurons in vitro

Discordant expression of Foxp3 in induced Tregs.

Fig. 6 Photoreceptor cell survival in the RCS rat retina after transplantation with hESC-RPE cell sheets. Photoreceptor cell survival in the RCS rat retina.

Modification of the βIII-tubulin C-terminal tail does not affect the incorporation of the protein into the microtubule network, the microtubule architecture,

Fig. 6 Combination therapy with LVSOD2 and LVshCTGF preserves flap volume and reduces fibrosis after RT. Combination therapy with LVSOD2 and LVshCTGF preserves.

V-SVZ cultures express transcription factors expected for region of origin. V-SVZ cultures express transcription factors expected for region of origin.

Establishment of monoclonal SMN2-GFP reporter line in HEK293.

Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7 cells or RPE cells. Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7.

Plk1 inhibition affects the NuMA turnover at the spindle pole.

Endogenous SMN1 is not recruited to stress granules in HeLa cells after diverse stresses. Endogenous SMN1 is not recruited to stress granules in HeLa cells.

Fig. 4 Lentiviral transgene expression penetrates tissue and provides durable effects in vivo. Lentiviral transgene expression penetrates tissue and provides.

Transcription factors defining dorsal or ventral identity are differentially expressed in dorsal and ventral hamartomas in TSC. Transcription factors defining.

Colocalization of the palmitoylation-deficient mutants of claudin-14 with the lysosomal membrane protein LAMP-2. Colocalization of the palmitoylation-deficient.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

Fig. 4. Detection of dFMR1 mRNA in dFMRP granules by FISH

(A) Faucher and Pliska hydrophobicity plot of D2SV protein sequence.

Identification of Z-FA-FMK as a potent compound that increases SMN protein expression in HEK293 and patient fibroblast cells. Identification of Z-FA-FMK.

MiR-200c/141 overexpression in LS-8 cells induces E-cad expression and cell-cell adhesion. miR-200c/141 overexpression in LS-8 cells induces E-cad expression.

Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and increased T-cell activation. Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and.

Fig. 3. Overexpression of wild-type GFP-CPAP, but not GFP-CPAP-377EE (GFP-377EE), induces cilia formation and promotes the growth of cilia.CAD cells (A,B)

ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

PTX-induced reporter protein expression in the brain of the IEG reporter line. PTX-induced reporter protein expression in the brain of the IEG reporter.

Characterization of NEX-CRE mice and analysis of Itgb1 expression by flow cytometry. a–n , Z/EG reporter mice carrying a CRE-inducible GFP transgene were.

LC8 and its binding partners display broad cellular localization.

Fig. 1. Rnd2 and Rnd3 induce stress fibres whereas Rnd1 reduces stress fibres in endothelial cells.(A) Rnd mRNAs are expressed in HUVECs. Total RNA was.

The BRCA1 aggregates exclude large nuclear structures.

Fibril-induced NM prions do not evolve from SGs.

RA signaling in early postnatal PFC

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

The regulatory domain of HSF1 is involved in the pro-apoptotic response to TNF. (A) Upper panel, functional domains and potential DAPK phosphorylation.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Neuronal and glial expression of Nogo-A and synaptic localization of Nogo-A and NgR 1 in the motor cortex. Neuronal and glial expression of Nogo-A and.

Subnuclear localization of HA-tagged EBNA1 protein.

Generation of an inducible form of FOXN1.

Co-expression of VSVG–Cdc42-CA, but not VSVG–Cdc42-DN with eGFP–gephyrin (green) and Myc–CB2ΔPH (blue) rescues postsynaptic gephyrin clustering, as shown.

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

PTZ-induced neuronal activity visualized by IEGs

Septin ring formation and chitin-containing septum formation are aberrant in filaments formed in response to staurosporine. Septin ring formation and chitin-containing.

S-Affs colocalize with SUMO in mammalian cells.

Brain ischemic tolerance model using mouse MCAO

Coexpression of Crf and Tac2 (A-P -1.5).

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

Fig. 3 C9ORF72 interacts with SMCR8 in a DENN domain–dependent manner.

Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

Presentation transcript:

Poly-GR mainly co-localizes with nucleolar ribosomes. Poly-GR mainly co-localizes with nucleolar ribosomes. (A) HEK293 cells were co-transfected with GFP, GFP-(GR)149, or (PR)175-GFP expression vectors and RFP-STAU1 as in Fig 3A and endogenous RPS6 detected by immunofluorescence. Note that the large cytoplasmic poly-GR/PR granules induced by STAU1 expression are not clearly stained with the ribosomal subunit compared with the cytoplasm. Merge shows additionally DAPI in gray. Single confocal planes are shown. Scale bar denotes 20 μm. (B) Frontal cortex of a C9orf72 patient and a control brain were co-stained for poly-GR and ribosomal proteins as in Fig 5A. RPS25 and RPL36A are partially sequestered into poly-GR inclusions. DAPI visualizes nuclei. Single confocal planes are shown. Scale bar depicts 20 μm. (C) Quantification of GR aggregates co-localizing with ribosomal proteins (n = 3 cortical sections with 100 GR aggregates counted each from two different patients; mean ± SEM is shown). Hannelore Hartmann et al. LSA 2018;1:e201800070 © 2018 Hartmann et al.