1. Co-expression of VSVG–Cdc42-CA, but not VSVG–Cdc42-DN with eGFP–gephyrin (green) and Myc–CB2ΔPH (blue) rescues postsynaptic gephyrin clustering, as shown by immunofluorescence staining of triple-transfected neurons (11+7 DIV) with synapsin-1 (red).
Co-expression of VSVG–Cdc42-CA, but not VSVG–Cdc42-DN with eGFP–gephyrin (green) and Myc–CB2ΔPH (blue) rescues postsynaptic gephyrin clustering, as shown by immunofluorescence staining of triple-transfected neurons (11+7 DIV) with synapsin-1 (red). (A) Myc–CB2ΔPH with no Cdc42. (A′) Postsynaptic gephyrin clusters are seen in dendrites (arrowheads) and on the soma upon the co-transfection of VSVG–Cdc42-CA. (A″) Gephyrin streaks and intrasomatic aggregates are readily evident upon co-transfection of VSVG–Cdc42-DN. Similar results were obtained in three independent experiments. Scale bar: 20 μm.
Shiva K. Tyagarajan et al. J Cell Sci 2011;124:
© 2011.