Regulation of Brain Cell Death and Survival After Cardiopulmonary Bypass Tatiana Zaitseva, PhD, Steven Schultz, MD, Gregory Schears, MD, Peter Pastuszko,

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Presentation transcript:

Regulation of Brain Cell Death and Survival After Cardiopulmonary Bypass Tatiana Zaitseva, PhD, Steven Schultz, MD, Gregory Schears, MD, Peter Pastuszko, MD, Scott Markowitz, MD, William Greeley, MD, David F. Wilson, PhD, Anna Pastuszko, PhD The Annals of Thoracic Surgery Volume 82, Issue 6, Pages 2247-2253 (December 2006) DOI: 10.1016/j.athoracsur.2006.06.024 Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Effect of deep hypothermic circulatory arrest (DHCA), low-flow cardiopulmonary bypass (LF), and selective cerebral perfusion (SCP) on cortical oxygen pressure in newborn piglets. The results are means ± SD from six experiments for each group, except DHCA group (n = 8). ap < 0.001 for significant difference from control values as determined by one-way analysis of variance, followed by the Wilcoxon signed-rank test. The Annals of Thoracic Surgery 2006 82, 2247-2253DOI: (10.1016/j.athoracsur.2006.06.024) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Effect of deep hypothermic circulatory arrest (DHCA), low-flow cardiopulmonary bypass (LFCPB), and selective cerebral perfusion (SCP) on B-cell leukemia protein (Bcl-2) and (N-terminal of Bcl-2-associated X protein (N-Bax) in the striatum of newborn piglets. (A) Representative Western blots for protein samples from DHCA, LFCPB, SCP, and control groups probed with N-Bax or Bcl-2 antibodies. (B) The means ± SD for DHCA (n = 8), LFCPB (n = 6), and SCP (n = 6) experiments, expressed in percentages to the corresponding values obtained for the control group of piglets (n = 6). ap < 0.02; bp < 0.01 for significant difference from control values as determined by one-way analysis of variance, followed by Mann-Whitney test. Statistical significance was set at p < 0.05. The Annals of Thoracic Surgery 2006 82, 2247-2253DOI: (10.1016/j.athoracsur.2006.06.024) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Effect of deep hypothermic circulatory arrest (DHCA), low-flow cardiopulmonary bypass (LFCPB), and selective cerebral perfusion (SCP) on total protein kinase B (Akt) and phosphorylated (pAkt) in the striatum of newborn piglets. (A) Representative Western blots for protein samples from DHCA, LFCPB, SCP, and control groups probed with antibodies against pAkt and total Akt. (B) The means ± SD for DHCA (n = 8), LFCPB (n = 6), SCP (n = 6) experiments are expressed in percentages to the corresponding values obtained for the control group of piglets (n = 6). ap < 0.05; bp < 0.01 for significant difference from control values as determined by one-way analysis of variance, followed by Mann-Whitney test. Statistical significance was set at p < 0.05. The Annals of Thoracic Surgery 2006 82, 2247-2253DOI: (10.1016/j.athoracsur.2006.06.024) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Effect of deep hypothermic circulatory arrest (DHCA), low-flow cardiopulmonary bypass (LFCPB), and selective cerebral perfusion (SCP) on total extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 (pERK1/2) in the striatum of newborn piglets. (A) Representative Western blots for protein samples from DHCA, LFCPB, SCP, and control groups probed with antibodies against pERK1/2 and tERK1/2). (B) The means ± SD for DHCA (n = 8), LFCPB (n = 6), SCP (n = 6) experiments are expressed in percentages to the corresponding values obtained for the control group of piglets (n = 6). ap < 0.05; bp < 0.02; cp < 0.01 for significant difference from control values as determined by one-way analysis of variance, followed by Mann-Whitney test. Statistical significance was set at p < 0.05. The Annals of Thoracic Surgery 2006 82, 2247-2253DOI: (10.1016/j.athoracsur.2006.06.024) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions