Klotho reduction causes macrophage invasion into the CP

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Klotho reduction causes macrophage invasion into the CP Klotho reduction causes macrophage invasion into the CP. (A–E) Cells expressing the macrophage markers MAC-2 (A, C, and D), IBA-1 (B), or LY6C (E) and Ly6c mRNA levels (F) in the CP of 19- to 24-mo-old WT vs. Klotho reduction causes macrophage invasion into the CP. (A–E) Cells expressing the macrophage markers MAC-2 (A, C, and D), IBA-1 (B), or LY6C (E) and Ly6c mRNA levels (F) in the CP of 19- to 24-mo-old WT vs. Klothoflox/flox (Flox) mice determined 9 mo after Cre injection. n = 4 to 9 mice per group. Sagittal CP sections were double-labeled for MAC-2 (red) and cytokeratin (gray) (A), IBA-1 (red) and Hoechst 33342 nuclear stain (blue) (B), or transthyretin (green) and MAC-2 (red) (C). [Scale bars: 100 µm (A), 50 µm (B), 25 μm (C).] *P < 0.05 by unpaired, two-tailed t test. Values in bar graphs are means ± SEM. Lei Zhu et al. PNAS 2018;115:48:E11388-E11396 ©2018 by National Academy of Sciences