1. Vegfa is overexpressed by E11
Vegfa is overexpressed by E11.5 in the Gpr124−/− forebrain and is associated with hypoxia.
Vegfa is overexpressed by E11.5 in the Gpr124−/− forebrain and is associated with hypoxia. (A) Real-time qPCR was used in a primary screen to evaluate the expression of Gpr124 and an additional 50 genes in Gpr124+/+ (WT) or Gpr124−/− (KO) forebrains microdissected at E13.5. Note that a mutant Gpr124 mRNA transcript is expressed in the KO, but its levels are ∼1% that of the WT transcript. (B) Vegfa is significantly overexpressed in Gpr124−/− brains by E11.5. *P < 0.02, **P < (C and D) VEGF mRNA expression (C, black) or hypoxia (D, green) is highest in the SVZ of the pallium opposite the isolectin-B4–labeled glomeruloids (red). (Scale bar, 100 μm.)‏
Mike Cullen et al. PNAS 2011;108:14:
©2011 by National Academy of Sciences