1. B cells infiltrate mouse and human pancreatic neoplasia and promote growth of KrasG12D-PDEC in vivo.
B cells infiltrate mouse and human pancreatic neoplasia and promote growth of KrasG12D-PDEC in vivo. A, IHC detection of B cells in human (CD20 staining) and mouse (B220 staining) in pancreata from hPanIN (20 patient samples), p48Cre (control, 5 mice), KC (10 mice), or KrasG12D-PDEC (9 mice) orthotopic lesions, as indicated. Inset, B cells in the parenchyma of an adjacent tissue section detected by immunofluorescence using anti-CD19 (green) and DAPI (blue). Representative images are shown. Scale bars, 100 μm. B, hematoxylin and eosin (H&E) staining and IHC staining for CD20, CXCL13, and vimentin from serial sections in a representative sample of human pancreatic cancer containing PanIN lesions. Inset, sections of human PanIN lesions were stained by immunofluorescence (CXCL13, red; vimentin, green; and DAPI, blue). Scale bars, 100 μm; inset, 7.5 μm. C, serial sections of a KC mouse pancreas were stained by IHC with CXCL13 or immunofluorescence (n = 10; CXCL13, red; vimentin, green; and DAPI, blue). A representative image is shown. Scale bars, 100 μm and 12 μm (inset). D, expression of Cxcl13 mRNA in cellular subsets isolated from pancreata of KC mice. Error bars indicate SD (n = 6). E, sections from orthotopic pancreatic grafts 2 weeks after GFP-KRasG12D-PDEC implantation into WT or μMT mice were stained with hematoxylin and eosin or anti-GFP antibody. Where indicated, μMT mice were reconstituted with WT B cells 2 days prior to orthotopic implantation. Representative images are shown. Scale bars, 100 μm. F, graph depicts quantification of the data in E and indicates the average fraction of GFP+ signal per field of view (FOV; 10 fields of view per animal; n = 12 WT, n = 14 μMT, n = 9 μMT+WT B cell animals). Error bars indicate SD; P values were determined by the Student t test (unpaired, two-tailed); ***, P <
Yuliya Pylayeva-Gupta et al. Cancer Discov 2016;6:
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