1. Altered cell migration factors and increased intestinal tumorigenesis in APC1638N/+ mice.
Altered cell migration factors and increased intestinal tumorigenesis in APC1638N/+ mice. (A) Increased intestinal tumor frequency is presented graphically showing higher tumorigenesis after 56Fe relative to sham and γ-radiation. (B) Immunoblot images showing increased EphB3 and β1-integrin expression and decreased Claudin1, Zo-1, and Map1b expression in tumor and adjacent normal tissues from APC1638N/+ mice. (C) Representative images of Par3 showing decreased expression in normal and tumor tissues after 56Fe radiation. (Scale bars, 10 μm.) (D) Immunofluorescence images showing decreased expression of E-cadherin in normal and tumor tissues after 56Fe radiation. (Scale bars, 10 μm.) (E) Representative images showing increased expression of Cyclin D1 in both normal and tumor tissues after 56Fe radiation. (Scale bars, 10 μm.) (F and G) Intestinal sections were stained using Alcian blue to score for mucin-producing goblet cells, a differentiated cell type in intestine, in normal and tumor tissues, and results are presented graphically showing decreased number after 56Fe radiation; *, significant relative to control; **, significant relative to γ-rays. Statistical significance is set at P < 0.05 and error bars represent mean ± SEM.
Santosh Kumar et al. PNAS 2018;115:42:E9832-E9841
©2018 by National Academy of Sciences