Inhibition of ILK by QLT-0267 reduces β-catenin accumulation and inhibits Snail1 expression in obstructive nephropathy. Inhibition of ILK by QLT-0267 reduces.

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Inhibition of ILK by QLT-0267 reduces β-catenin accumulation and inhibits Snail1 expression in obstructive nephropathy. Inhibition of ILK by QLT-0267 reduces β-catenin accumulation and inhibits Snail1 expression in obstructive nephropathy. (A) Representative Western blot demonstrates that QLT-0267 administration significantly reduced β-catenin abundance in the obstructed kidney at 7 d after UUO. Numbers denote each individual mouse in a given group. (B) Graphic presentation shows the relative abundance of β-catenin in various groups as indicated. Data are means ± SEM of five animals per group. *P < 0.05 versus sham controls; †P < 0.05 versus vehicle controls. (C through E) Immunohistochemical staining shows β-catenin localization in different groups. (C) Sham controls. (D) UUO 7d. (E) UUO 7d injected with QLT-0267. Bar = 40 μm. Arrowheads indicate nuclear staining of β-catenin. (F) Quantitative determination of renal Snail1 mRNA levels by real-time RT-PCR in various groups as indicated. Relative renal Snail1 mRNA levels (fold induction versus sham controls) were presented. *P < 0.05 versus sham controls; †P < 0.05 versus vehicle controls. The dosages of QLT-0267 (mg/kg body wt) are given. Yingjian Li et al. JASN 2009;20:1907-1918 ©2009 by American Society of Nephrology