MICROGRAPH ANALYSIS LAB In lab book Create 5 tables based on the following prompts and provided micrograph images Be sure each table has a clear title and column headings

TABLE 1: Sort which electron-micrographs are from a prokaryotic cell and which are from a eukaryotic cell Put the letter of the micrograph image into the table. Get a stamp when done with table 1.

TABLE 2: sort and identify eukaryotic images as being plant or animal Put the letter of the micrograph image into the table. Get a stamp when done with table 2.

TABLE 3: create a table in which you list the cellular structures shown in the eukaryotic images PICTURE LIST OF STRUCTURES VISIBLE H HINT: 2 I HINT: 4 J K HINT: 3 L M HINT: 1 O PICTURE LIST OF STRUCTURES VISIBLE B HINT: 6 C HINT: 3 D HINT: 4ish E HINT: 3ish F HINT: 2 G list organelles visible. Get a stamp when done with table 3.

TABLE 4: Now that you are an expert at identifying structures TABLE 4: Now that you are an expert at identifying structures...see if you can predict cellular FUNCTION given these micrograph images. PICTURE # FUNCTION OF CELL STRUCTURAL EVIDENCE of FUNCTION Cell lining mammal small intestine Cell of pancreas that secretes insulin (a protein hormone) Cell of leaf responsible for photosynthesis Immune system cell responsible for engulfing invading bacteria Explain why you think each cell has the function you matched. Get a stamp when done with table 4.

Please show your working in your lab book. How much larger than life size is the picture? TABLE 5: Calculate image magnification for micrographs 1, 2, 3 and 4 given the information provided in the table. Please show your working in your lab book. Get a stamp when done with table 5. Check and correct your answers PICTURE IMAGE MAGNIFICATION A Calculate using scale bar in image. B Calculate knowing that cell nucleus is 10um in width. C Calculate knowing that an average mitochondria is 1 um in width. D Calculate knowing that the typical chloroplast is 6 um in length.

DRAWINGS Look at the following two electron micrographs carefully and examine the significant features that will be included in the drawing. DRAW ONLY WHAT YOU SEE!! Do not include what you think you should see. All drawings must be done in pencil ONLY. Drawings must be large and clear so that features can be easily distinguished. Use at least ½ page for each drawing. No more than two drawings should be on a single page of unlined white paper. Always use distinct, single lines when drawing. All drawings must have the following indicated: Title (give a full, clear and concise title that explains what is being illustrated) Magnification (indicate the magnification at which the specimen was drawn) Labels (each label line must be straight and should not overlap with other label lines; all labels must be to one side and aligned in a vertical column) Scale (always include a scale bar indicating the length or width of the specimen drawn)

Drawing sample 1: “Typical Homo sapiens Pancreas Beta Cell” Observe the cells in the micrograph image. Draw a single cell on unlined white paper. Title the drawing. Label structures (note: darkest circles are secretory vesicles that will secrete insulin out of the cell and into the bloodstream). Add a scale bar to indicate that the cell is 10 um long. (Source) Calculate the drawing magnification.

Drawing sample 2: “Typical Zea mays Leaf Cell” Observe the cell in the micrograph image. Draw a single cell on unlined white paper. Title the drawing. Label structures Add a scale bar to indicate the length of the cell. Calculate the drawing magnification.