1. Warm-Up (10/29)
On the piece of white paper from the back, answer the following question.
Name
Date
Period
Insulin is a protein hormone released into the bloodstream by the pancreas to regulate blood glucose (sugar) levels. Describe how insulin is secreted by pancreatic cells. Use at least four organelles, including the nucleus, in your description.

2. Bacterial Transformation
artificial DNA plasmid
E. coli bacteria
Transformed E. coli

3. Bacterial Transformation

4. Bacterial Transformation
Gene you put in

5. Bacterial Transformation
No ampillicin – smear of lots of bacteria
Added ampicillin - only transformed bacteria grow

6. Bacterial Transformation
No arabinose – no GFP genes activated
Added arabinose – GFP genes activated

7. Bacterial Transformation
Can replace with insulin for diabetes patients

8. Critical Thinking Question
Scientists in a lab have the capacity to purify RNA from the human insulin gene.
Describe how they would have to make DNA from the RNA, and once they cut open a plasmid, how they are able to glue the insulin DNA into it.

9. Closure (10/29)
On the piece of white paper from the back, answer the following question:
Name
Date
Period
Explain the difference, and the importance, between ampicillin and arabinose in a bacterial transformation experiment.
Scale 1 – 10