Science Learning Center Chemistry Pre-laboratory Tutorials Written By Patrick Herklotz

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Previous Slide Replay Continue Tutorial 3 Calibrating the Vernier Colorimeter Sensor and Recording Data Using this Sensor

Top View Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 1: Assemble the LabPro Calculator as explained in Tutorial 1

Top View Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 1: Assemble the LabPro Calculator as explained in Tutorial 1

Top View Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Side View Channel 1Channel 2Channel 3Channel 4 Step 2: Obtain the colorimeter sensor and plug it into channel 1. You must run the DataMate program to turn the colorimeter on. The colorimeter will measure absorbance in a default wavelength of 470nm. A green light illuminates to signify which wavelength is selected. Allow the colorimeter to warm up for 5 minutes before beginning any experiment.

CAL 430nm 470nm565nm 635nm Top View Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 2: Obtain the colorimeter sensor and plug it into channel 1. You must run the DataMate program to turn the colorimeter on. The colorimeter will measure absorbance in a default wavelength of 470nm. A green light illuminates to signify which wavelength is selected. Allow the colorimeter to warm up for 5 minutes before beginning any experiment. Side View Channel 1Channel 2Channel 3Channel 4 Channel 1 Port click VERNIER SOFTWARE DATAMATE (VER 1.15) ROM 6.27 (C)2003 Checking Sensors Mode: TIME GRAPH-180 1: SETUP 4: ANALYZE 2: START 5: TOOLS 3: GRAPH 6: QUIT CH 1: Absorbance Mode: TIME GRAPH-180 1: SETUP 4: ANALYZE 2: START 5: TOOLS 3: GRAPH 6: QUIT

Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) CAL 430nm 470nm Step 3: Fill a Cuvette full of distilled water and place it into the colorimeter. Be sure that the clear side of the Cuvette is facing forward and backward in the colorimeter and the striated sides face to the side. Push the > key on the colorimeter and hold it down until the green light changes to 565nm. This signifies that this new wavelength has been selected.

Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 3: Fill a Cuvette full of distilled water and place it into the colorimeter. Be sure that the clear side of the Cuvette is facing forward and backward in the colorimeter and the striated sides face to the side. Push the > key on the colorimeter and hold it down until the green light changes to 565nm. This signifies that this new wavelength has been selected. CAL 430nm 470nm565nm 635nm

CAL 430nm 470nm565nm 635nm Top View Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) TI-84 Silver Edition Texas Instruments CH 1: Absorbance Mode: TIME GRAPH-180 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT Step 4: To calibrate the colorimeter, press the 1 key (SETUP). In the next menu, press the 3 key (ZERO). In the third menu press the 2 key (Zero ALL CHANNELS). In the final menu, press the ENTER key.

CAL 430nm 470nm565nm 635nm Top View Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 4: To calibrate the colorimeter, press the 1 key (SETUP). In the next menu, press the 3 key (ZERO). In the third menu press the 2 key (Zero ALL CHANNELS). In the final menu, press the ENTER key. TI-84 Silver Edition Texas Instruments CH 1: Absorbance Mode: TIME GRAPH-180 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT CH 1: Colorimeter CH 2: CH 3: CH 4: DIG1: DIG2: MODE: TIME GRAPH-900 1:OK 3:ZERO 2:CALIBRATE 4:SAVE/LOAD SELECT CHANNEL 1: CH1-ABSORBANCE 2: ALL CHANNELS CH 1: ABSORBANCE PRESS [ENTER] TO ZERO CH 1: ABSORBANCE 0 Mode: TIME GRAPH-900 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT Colorimeter is now Calibrated!

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) TI-84 Silver Edition Texas Instruments CH 1: ABSORBANCE CH 2: CH 3: CH 4: DIG1: DIG2: MODE: TIME GRAPH-180 1:OK 3:ZERO 2:CALIBRATE 4:SAVE/LOAD Step 5: Change the Data Collection mode first by pressing the 1 key (SETUP). In the next menu, use the down key to scroll the cursor to the left of MODE; press the ENTER key. In the SELECT MODE menu, press the 3 key to select EVENTS WITH ENTRY. Press 1 in the final menu to go back to the default screen.

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 5: Change the Data Collection mode first by pressing the 1 key (SETUP). In the next menu, use the down key to scroll the cursor to the left of MODE; press the ENTER key. In the SELECT MODE menu, press the 3 key to select EVENTS WITH ENTRY. Press 1 in the final menu to go back to the default screen. TI-84 Silver Edition Texas Instruments CH 1: Absorbance Mode: TIME GRAPH-990 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT CH 1: ABSORBANCE CH 2: CH 3: CH 4: DIG1: DIG2: MODE: TIME GRAPH-180 1:OK 3:ZERO 2:CALIBRATE 4:SAVE/LOAD SELECT MODE 1:LOGDATA 2:TIME GRAPH 3:EVENTS WITH ENTRY 4:SINGLE POINT 5:SELECTED EVENTS 6:RETURN TO SETUP SCREEN CH 1: ABSORBANCE CH 2: CH 3: CH 4: DIG1: DIG2: MODE: TIME GRAPH-180 1:OK 3:ZERO 2:CALIBRATE 4:SAVE/LOAD CH 1: ABSORBANCE 0 Mode: EVENTS WITH ENTRY 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) TI-84 Silver Edition Texas Instruments CAL 430nm 470nm565nm 635nm Cuvette A CH 1: ABSORBANCE 0 Mode: EVENTS WITH ENTRY 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT Step 6: Begin collecting data by pressing the 2 key (START) in the default menu. Be sure to use a chemwipe to get all the fingerprints off the outside of the cuvette you are using. Place cuvette A into the colorimeter. Give the Colorimeter 15 seconds to begin taking accurate readings.

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 6: Begin collecting data by pressing the 2 key (START) in the default menu. Be sure to use a chemwipe to get all the fingerprints off the outside of the cuvette you are using. Place cuvette A into the colorimeter. Give the Colorimeter 15 seconds to begin taking accurate readings. TI-84 Silver Edition Texas Instruments CAL 430nm 470nm565nm 635nm Cuvette A CH 1: ABSORBANCE 0 Mode: EVENTS WITH ENTRY 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT PRESS [ENTER] TO COLLECT OR [STO] TO STOP

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 7: After 15 seconds press the ENTER key. In the next menu, you will be prompted to ENTER VALUE? Type in the concentration of the substance in cuvette A and press the ENTER key. Pull Cuvette A out and repeat steps 6 and 7 for the rest of the substances in separate cuvettes. TI-84 Silver Edition Texas Instruments CAL 430nm 470nm565nm 635nm PRESS [ENTER] TO COLLECT OR [STO] TO STOP

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 7: After 15 seconds press the ENTER key. In the next menu, you will be prompted to ENTER VALUE? Type in the concentration of the substance in cuvette A and press the ENTER key. Pull Cuvette A out and repeat steps 6 and 7 for the rest of the substances in separate cuvettes. TI-84 Silver Edition Texas Instruments CAL 430nm 470nm565nm 635nm PRESS [ENTER] TO COLLECT OR [STO] TO STOP ENTER VALUE ? PRESS [ENTER] TO COLLECT OR [STO] TO STOP

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) TI-84 Silver Edition Texas Instruments CAL 430nm 470nm565nm 635nm PRESS [ENTER] TO COLLECT OR [STO] TO STOP Step 7: Once you have recorded the absorbance of the substances in cuvette A, B, and C using steps 6 and 7, press the STO button to end recording additional measurements. A stat plot of the recorded absorbencies will appear on the screen. Press ENTER to escape this screen and return to the default menu.

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 7: Once you have recorded the absorbance of the substances in cuvette A, B, and C using steps 6 and 7, press the STO button to end recording additional measurements. A stat plot of the recorded absorbencies will appear on the screen. Press ENTER to escape this screen and return to the default menu. TI-84 Silver Edition Texas Instruments CAL 430nm 470nm565nm 635nm PRESS [ENTER] TO COLLECT OR [STO] TO STOP ABSORBANCE P1 X=concentration # y=Absorbance CH 1: ABSORBANCE Mode: EVENTS WITH ENTRY 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 8: Press the 6 key (QUIT) then the ENTER key to save your recorded concentration vs. absorbance data in L1 and L2. The data can be downloaded the into the LoggerPro program as described in tutorial 2. TI-84 Silver Edition Texas Instruments CAL 430nm 470nm565nm 635nm CH 1: ABSORBANCE Mode: EVENTS WITH ENTRY 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT

CAL 430nm 470nm565nm 635nm Previous Slide Replay Continue VERNIER LabPro Quick Setup Start/Stop Transfer Vernier Power Supply STAT PLOT F1 TBLSET F2 FORNAT F3CALC F4 TABLE F5 Alpha 2ND Mode DEL STAT PRGM COS ( X,T,O,N APPS MATH (-) x + - ÷ Enter X -1 X2X2 LOG LN STO> ON SIN, VARS CLEAR TAN > ) Step 8: Press the 6 key (QUIT) then the ENTER key to save your recorded concentration vs. absorbance data in L1 and L2. The data can be downloaded the into the LoggerPro program as described in tutorial 2. TI-84 Silver Edition Texas Instruments CAL 430nm 470nm565nm 635nm CH 1: ABSORBANCE Mode: EVENTS WITH ENTRY 1: SETUP4: ANALYZE 2: START5: TOOLS 3: GRAPH6: QUIT EVENTS IN L1 CH1 IN L2 CH2 IN L3 CH3 IN L4 CH4 IN L5 D IN L6, V In L7 A IN L8 [ENTER]

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