Therapeutic Effect of Midkine on Cardiac Remodeling in Infarcted Rat Hearts Shinya Fukui, MD, Satoru Kitagawa-Sakakida, MD, PhD, Sin Kawamata, MD, PhD,

Slides:

Advertisements

Similar presentations

Date of download: 6/9/2016 Copyright © The American College of Cardiology. All rights reserved. From: Cardiomyocyte-Specific Deletion of Gsk3α Mitigates.

Advertisements

Date of download: 6/23/2016 Copyright © The American College of Cardiology. All rights reserved. From: 17-Beta-Estradiol increases cardiac remodeling and.

Myocardial functional recovery after fibroblast growth factor 2 gene therapy as assessed by echocardiography and magnetic resonance imaging Keith A Horvath,

Results of Initial Operations in Non–Small Cell Lung Cancer Patients With Single-Level N2 Disease Yasuhiko Ohta, MD, Yosuke Shimizu, MD, Hiroshi Minato,

Paracrine Action Enhances the Effects of Autologous Mesenchymal Stem Cell Transplantation on Vascular Regeneration in Rat Model of Myocardial Infarction

Cell transplantation to prevent heart failure: a comparison of cell types Takeshiro Fujii, MD, Terrence M Yau, MD, MS, Richard D Weisel, MD, Nobuhisa.

Uswa Shahzad, BHSc, Guangming Li, MD, Yaoguang Zhang, MD, Terrence M

Therapeutic angiogenesis with intramyocardial administration of basic fibroblast growth factor Michio Kawasuji, MD, Hiroshi Nagamine, MD, Masahiro Ikeda,

Direct epicardial shock wave therapy improves ventricular function and induces angiogenesis in ischemic heart failure Daniel Zimpfer, MD, Seyedhossein.

Cardiomyocyte transplantation does not reverse cardiac remodeling in rats with chronic myocardial infarction Yutaka Sakakibara, MD, Keiichi Tambara,

Late Complications of Gelatin-Resorcin-Formalin Glue in the Repair of Acute Type A Aortic Dissection Hiroki Hata, MD, PhD, Hiroshi Takano, MD, PhD, Goro.

Repair of Myocardial Infarction by Epicardial Deposition of Bone Marrow Cell-Coated Muscle Patch in a Murine Model Laurent Barandon, MD, Thierry Couffinhal,

Cardiomyocyte Transplantation Improves Heart Function

Optimal time for cardiomyocyte transplantation to maximize myocardial function after left ventricular injury Ren-Ke Li, MD, PhD, Donald A.G. Mickle,

Cell transplantation to prevent heart failure: a comparison of cell types Takeshiro Fujii, MD, Terrence M Yau, MD, MS, Richard D Weisel, MD, Nobuhisa.

Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac.

Cell-based gene therapy modifies matrix remodeling after a myocardial infarction in tissue inhibitor of matrix metalloproteinase-3–deficient mice Denis.

Paracrine Action Enhances the Effects of Autologous Mesenchymal Stem Cell Transplantation on Vascular Regeneration in Rat Model of Myocardial Infarction

An ovine model of postinfarction dilated cardiomyopathy

Allogeneic Mesenchymal Precursor Cell Therapy to Limit Remodeling After Myocardial Infarction: The Effect of Cell Dosage Hirotsugu Hamamoto, MD, Joseph.

Sustained Benefits of the CorCap Cardiac Support Device on Left Ventricular Remodeling: Three Year Follow-up Results From the Acorn Clinical Trial Randall.

A slow-releasing form of prostacyclin agonist (ONO1301SR) enhances endogenous secretion of multiple cardiotherapeutic cytokines and improves cardiac function.

Mesenchymal stem cell implantation in a swine myocardial infarct model: engraftment and functional effects Jay G. Shake, MD, Peter J. Gruber, MD, PhD,

Liam P. Ryan, MD, Kanji Matsuzaki, MD, PhD, Mio Noma, MD, Benjamin M

Combined Strategy Using Myoblasts and Hepatocyte Growth Factor in Dilated Cardiomyopathic Hamsters Haruhiko Kondoh, MD, Yoshiki Sawa, MD, Norihide Fukushima,

Reorganization of cytoskeletal proteins and prolonged life expectancy caused by hepatocyte growth factor in a hamster model of late-phase dilated cardiomyopathy

Combined transplantation of skeletal myoblasts and angiopoietic progenitor cells reduces infarct size and apoptosis and improves cardiac function in chronic.

Changfa Guo, MD, Husnain Kh

Malignant B-Cell Lymphoma Arising in a Large, Left Atrial Myxoma

Biodegradable vs Nonbiodegradable Cardiac Support Device for Treating Ischemic Cardiomyopathy in a Canine Heart Mutsunori Kitahara, MD, Shigeru Miyagawa,

Pravastatin Improves Remodeling and Cardiac Function After Myocardial Infarction by an Antiinflammatory Mechanism Rather than by the Induction of Angiogenesis

A novel vascularized patch enhances cell survival and modifies ventricular remodeling in a rat myocardial infarction model Qi Zhou, MD, PhD, Jian-Ye.

Volume 23, Issue 2, Pages (June 2016)

Targeted overexpression of leukemia inhibitory factor to preserve myocardium in a rat model of postinfarction heart failure Mark F. Berry, MD, Timothy.

Volume 10, Issue 2, Pages (February 2018)

Marrow Stromal Cells as Universal Donor Cells for Myocardial Regenerative Therapy: Their Unique Immune Tolerance Rony Atoui, MD, MS, Juan-Francisco Asenjo,

Therapeutic angiogenesis induced by local autologous bone marrow cell implantation Kimikazu Hamano, MD, Tao-Sheng Li, MD, Toshiro Kobayashi, MD, Ken Hirata,

Vascular Endothelial Growth Factor Prevents Endothelial-to-Mesenchymal Transition in Hypertrophy Ben M.-W. Illigens, MD, Alejandra Casar Berazaluce,

Therapeutic Potential of Human Umbilical Cord Derived Stem Cells in a Rat Myocardial Infarction Model Kai Hong Wu, MD, PhD, Bin Zhou, PhD, Cun Tao Yu,

Proteasome Inhibition Attenuates Infarct Size and Preserves Cardiac Function in a Murine Model of Myocardial Ischemia-Reperfusion Injury William E. Stansfield,

Improvement of cardiac function in the failing rat heart after transfer of skeletal myoblasts engineered to overexpress placental growth factor Matthias.

Cardiac dysfunction induced by experimental myocardial infarction impairs the host defense response to bacterial infection in mice because of reduced.

Left Ventricular Reconstructive Surgery in Ischemic Dilated Cardiomyopathy Complicated With Cardiogenic Shock Koji Takeda, MD, Goro Matsumiya, MD, Hajime.

Novel regenerative therapy using cell-sheet covered with omentum flap delivers a huge number of cells in a porcine myocardial infarction model Yasuhiro.

Injectable Microsphere Gel Progressively Improves Global Ventricular Function, Regional Contractile Strain, and Mitral Regurgitation After Myocardial.

Improved heart function with myogenesis and angiogenesis after autologous porcine bone marrow stromal cell transplantation Shinji Tomita, MD, PhD, Donald.

Modification of Infarct Material Properties Limits Adverse Ventricular Remodeling Masato Morita, MD, Chad E. Eckert, PhD, Kanji Matsuzaki, MD, PhD, Mio.

Skeletal myoblast sheet transplantation improves the diastolic function of a pressure- overloaded right heart Takaya Hoashi, MD, Goro Matsumiya, MD, PhD,

Complete Revascularization of the Three-Vessel Territories Using a Left Internal Thoracic Artery Composite Graft Ho Young Hwang, MD, PhD, Hong Chul Oh,

Role of Surgical Ventricular Restoration in the Treatment of Ischemic Cardiomyopathy Jun Liu, MD, Zixiong Liu, MD, Qiang Zhao, MD, Anqing Chen, MD, Zhe.

Significant improvement of heart function by cotransplantation of human mesenchymal stem cells and fetal cardiomyocytes in postinfarcted pigs Jiang-Yong.

Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart Naosumi Sekiya, MD, Goro Matsumiya, MD, PhD, Shigeru.

Restoration of left ventricular geometry and improvement of left ventricular function in a rodent model of chronic ischemic cardiomyopathy Jiashing Yu,

Sublocalization of Cardiac Involvement in Sarcoidosis and Surgical Exclusion in Patients With Congestive Heart Failure Masanori Hirota, MD, PhD, Minoru.

Association of electrostimulation with cell transplantation in ischemic heart disease Abdel Shafy, MD, Thomas Lavergne, MD, Christian Latremouille, MD,

Successful Mitral Valve Replacement in a Patient With Ehlers-Danlos Syndrome Type VI Hiroshi Takano, MD, Yuji Miyamoto, MD, Yoshiki Sawa, MD, Norihide.

Elimination of Ischemic Mitral Regurgitation Does Not Alter Long-Term Left Ventricular Remodeling in the Ovine Model Kanji Matsuzaki, MD, PhD, Masato.

Prevascularization with gelatin microspheres containing basic fibroblast growth factor enhances the benefits of cardiomyocyte transplantation Yutaka.

Pedunculated Endocardial Left Ventricular Fibroma Presenting With Cerebral and Bilateral Peripheral Embolization Ahmad K. Darwazah, FRCS, Jamal Shoeb,

Grafted skeletal myoblast sheets attenuate myocardial remodeling in pacing-induced canine heart failure model Hiroki Hata, MD, Goro Matsumiya, MD, PhD,

Volume 21, Issue 3, Pages (March 2013)

Myocardial functional recovery after fibroblast growth factor 2 gene therapy as assessed by echocardiography and magnetic resonance imaging Keith A Horvath,

Limiting neutrophilic inflammation during active phase (ZT13) reduces MI damage Limiting neutrophilic inflammation during active phase (ZT13) reduces MI.

Advancing Cor Triatriatum in Fontan Circulation

Survival After Isolated Coronary Artery Bypass Grafting in Patients With Severe Left Ventricular Dysfunction Waleed A. Ahmed, MD, Phillip J. Tully, BHSc(Hons),

Vascular Effects of Cyclosporin A and Acute Rejection in Canine Heart Transplantation Denis Bouchard, MD, Marc-Antoine Despatis, MD, Josie Buluran, BSc,

Results of Initial Operations in Non–Small Cell Lung Cancer Patients With Single-Level N2 Disease Yasuhiko Ohta, MD, Yosuke Shimizu, MD, Hiroshi Minato,

Andrew D Cochrane, Shobhna Pathik, Joseph J Smolich, Robert A

Development of abnormal tissue architecture in transplanted neonatal rat myocytes Peter Whittaker, PhD, Jochen Müller-Ehmsen, MD, Joan S Dow, BS, Larry.

Gene transfection of hepatocyte growth factor attenuates the progression of cardiac remodeling in the hypertrophied heart Keiji Iwata, MD, Yoshiki Sawa,

Presentation transcript:

Therapeutic Effect of Midkine on Cardiac Remodeling in Infarcted Rat Hearts Shinya Fukui, MD, Satoru Kitagawa-Sakakida, MD, PhD, Sin Kawamata, MD, PhD, Goro Matsumiya, MD, PhD, Naomasa Kawaguchi, PhD, Nariaki Matsuura, MD, PhD, Yoshiki Sawa, MD, PhD The Annals of Thoracic Surgery Volume 85, Issue 2, Pages 562-570 (February 2008) DOI: 10.1016/j.athoracsur.2007.06.002 Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 (A) Expression of midkine (MDK) mRNA in infarcted rat hearts. Open symbols represent the infarcted left ventricular myocardium, and closed symbols represent the noninfarcted myocardium of ligated left anterior descending artery (LAD) Lewis hearts. (B) Immunohistochemical staining for MDK protein showed MDK in the surviving cardiomyocytes of the border zone and infarcted area (arrowhead) but not in the noninfarcted area of the heart after myocardial infarction. Left, border-zone area in the hematoxylin-eosin–stained heart; middle, border-zone area in the anti-MDK–stained heart; right, noninfarcted area in the anti-MDK-stained heart. The Annals of Thoracic Surgery 2008 85, 562-570DOI: (10.1016/j.athoracsur.2007.06.002) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Echocardiographic assessments of cardiac function after midkine (MDK) treatment. Changes in left ventricular (LV) end-diastolic area (A), LV end-systolic area (B), or ejection fraction (C) from baseline measurements for the groups treated with gel alone (open symbols) or with 25 μg recombinant MDK (closed symbols). Repeated measurement analysis of variance indicated that the two curves were significantly different (A, p < 0.01; B, p < 0.05; C, p < 0.01), and the comparisons at each time point also indicated significant differences (*p < 0.05; **p < 0.01 versus control [Cont.]). This attenuation of LV remodeling was dose dependent, as judged from the change in LV end-diastolic area 6 weeks after treatment (D) (*p < 0.05 versus control). The Annals of Thoracic Surgery 2008 85, 562-570DOI: (10.1016/j.athoracsur.2007.06.002) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Morphometric analysis of infarcted rat hearts after midkine (MDK) treatment. Hearts treated with gel alone or with 25 μg MDK were stained with Masson’s trichrome, and two representative sections from each group are shown. (A) Left, gel-alone treated control; right, MDK treated. Note the smaller left ventricle cavity and thicker infarct scar and left ventricular muscle in the MDK-treated hearts. (B, C) The thickness and the length of the myocardial infarction (MI) area were thicker and shorter in the MDK-treated hearts. (D) The infarct scar area was not significantly affected by any dose of MDK injected. (E) The area of the noninfarcted muscle was dose-dependently increased by the injection of MDK. (Each group, n = 10.) (EF = ejection fraction; LVEDA = left ventricular end-diastolic area; W = weeks.) The Annals of Thoracic Surgery 2008 85, 562-570DOI: (10.1016/j.athoracsur.2007.06.002) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Assessment of the collagen volume fraction (CVF) in the gel-treated control and midkine (MDK)-treated hearts. Sections of heart were stained with picro-sirius red staining. Infarcted area in the MDK-treated hearts showed striking accumulation of collagen (A, upper left: gel-alone treated control; upper right: MDK-treated in the infarcted area). The gel-treated control hearts revealed homogeneous collagen volume fraction in their noninfarcted myocardium (A, lower left: gel-treated control; lower middle: MDK-treated in the noninfarcted area; lower right: age-matched normal rat hearts). (B) Left, the collagen volume fraction in the infarcted area was significantly greater in the MDK-treated hearts than in the gel-treated control hearts. Right, the collagen volume fraction in the MDK-treated hearts was significantly less in the noninfarcted area. (MI = myocardial infarction.) The Annals of Thoracic Surgery 2008 85, 562-570DOI: (10.1016/j.athoracsur.2007.06.002) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 5 Assessment of vascular density in the gel-treated control and midkine (MDK)-treated hearts. (A) Sections of heart were stained with an antibody to von Willebrand factor, and most of the positive vessels were observed in the infarct scar area or the border zone of the MDK-treated hearts (upper: low magnification; lower: high magnification). (B) The vascular density in the MDK-treated hearts was more than twice that in the gel-treated control hearts. Arrowheads indicate von Willebrand factor-positive vessels. The Annals of Thoracic Surgery 2008 85, 562-570DOI: (10.1016/j.athoracsur.2007.06.002) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 6 Assessment of cardiac myocyte remodeling in the gel-treated control and midkine (MDK)-treated hearts. (A) Hearts treated with gel alone or 25 μg MDK were periodic acid-Schiff stained. Age-matched normal Lewis hearts were stained for comparison. The cardiomyocyte width (B), length (C), and cell size (D) were measured. The cardiomyocytes of the MDK-treated hearts were significantly less hypertrophic than those of the gel-treated control hearts, although the cells were still significantly hypertrophic compared with the normal cardiomyocytes. The Annals of Thoracic Surgery 2008 85, 562-570DOI: (10.1016/j.athoracsur.2007.06.002) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions