1. Repair of Myocardial Infarction by Epicardial Deposition of Bone Marrow Cell-Coated Muscle Patch in a Murine Model 
Laurent Barandon, MD, Thierry Couffinhal, MD, PhD, Pascale Dufourcq, PhD, Philippe Alzieu, BS, Danièle Daret, BS, Claude Deville, MD, Cécile Duplàa, PhD 
The Annals of Thoracic Surgery 
Volume 78, Issue 4, Pages (October 2004)
DOI: /j.athoracsur

2. Fig 1
Epicardial deposition of bone marrow cells coated on an abdominal muscle patch. (a) Thirty days after myocardial infarction induction by cryoinjury, an abdominal muscle patch is dissected and then cupped, by means of a purse-string suture, to adapt its shape to the left ventricular geometry and form a “cell tank” in which to deposit bone marrow cells. (b) Bone marrow cells (5 × 106) are mixed into a collagen-rich matrix and then deposited on the patch. (c) The bone marrow cell–coated patch is sutured onto the cryoinfarcted zone. (d) The bone marrow cell–coated muscle patch is shown wrapped around the infarcted zone, 15 days after patch implantation. (Masson's trichrome staining, magnification ×10.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )

3. Fig 2
Angiogenesis and bone marrow cell proliferation in myocardial infarction scar tissue. Transplanted bone marrow cells that have migrated into the scar are demonstrated by β-galactosidase (βGal) immunostaining (brown). The bone marrow cells represent 8% of the total number of cells in the scar. Improved capillary density and cell proliferation within the myocardial infarction scar in patch + bone marrow cell–treated mice are demonstrated by CD31 staining (for endothelial cells) and bromide-deoxyuridine (BrdU) staining (for proliferating cells). (Ac = antibody.) (Magnification ×40.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )

4. Fig 3
Effect of bone marrow cells on capillary density (a), cell proliferation (b), and scar thickness (c) within myocardial infarction scar tissue. *p less than (BrdU = bromide-deoxyuridine.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )

5. Fig 4
Effects of different treatments on cardiac function and remodeling. (a) Effect on infarct size. Note that infarct size was not reduced by either patch-only treatment or patch + bone marrow cell treatment. (b) Effect on diastolic distention. Note that left ventricular cavity area was reduced in both patch-only–treated and patch + bone marrow cell–treated mice, as compared with sham-treated mice. **p < (c) Effect on cardiac function. As measured by the maximum rate of increase of left ventricular pressure (Dp/Dt Max), patch + bone marrow cell treatment resulted in an amplified contractile index. *p < 0.05; **p < 0.01; ***p < (LV = left ventricular; NS = not significant.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )

6. Fig 5
Angiogenesis and bone marrow cell proliferation in muscle patch. Transplanted bone marrow cells that have migrated into the muscle patch are detected by β-galactosidase (βGal) staining (brown). The bone marrow cells represent 7% of the total number of cells in the muscle patch. Improved capillary density and cell proliferation in the bone marrow cell–coated muscle patch are demonstrated by CD31 staining (for endothelial cells) and bromide-deoxyuridine (BrdU) staining (for proliferating cells), respectively. (Ac = antibody.) (Magnification ×40.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )

7. Fig 6
Effect of bone marrow cells on capillary density (a), cell proliferation (b), and scar thickness (c) within muscle patch. *p < 0.05; **p < (BrdU = bromide deoxyuridine.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )