The Alfin-like PHD Zinc Finger Transcription Factor Family in Arabidopsis Thaliana Melissa Borkenhagen 8 May 2006 HC70AL DR. Bob Goldberg

Gene AT5G20510 T-DNA 5’ UTR EXON 3’ UTR INTRON Chromosome 5 LBb1 FW T-DNA INSERT ACCORDING TO SALK Chromosome 5

Gene AT3G42790 T-DNA 5’ UTR EXON 3’ UTR INTRON Chromosome 3 LBb1 FW T-DNA INSERT ACCORDING TO SALK Chromosome 3

In which plant organs are the genes active? Gene AT5G20510 Gene AT3G42790 Consistent with GeneChip Data, both genes appear to be active in these plant organs.

Gene AT5G20510 Plant Genotypes Separation of primers Only one T-DNA Insert Plant 1 is the only Homozygous Mutant Plant Strange double bands What are the genotypes?

Gene AT3G42790 Plant Genotypes Plants 1 and 4 are Homozygous Mutants Plant 5: Heterozygous?

Are there any observable phenotypic differences between wild type and mutant plants? No significant differences observed between wild type and mutant plants.

Is continued work on this family of transcription factors important? Research has shown that members of the alfin-like PHD zinc finger transcription factor family may be influential in plant development. Some appear to be particularly influential during seed development, with a possible role in the differential seedling development in darkness and light. Thus, continued work on these proteins is important!