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HC70AL Research Presentation

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Presentation on theme: "HC70AL Research Presentation"— Presentation transcript:

1 HC70AL Research Presentation
Eric Newton

2 First gene AT3G47640 Wild Type: 1,409 Mutant: 730

3 Second gene AT3G61310 Wild Type: 621 Mutant: 609

4 First gene plants genotype
Plant 1: Heterozygous Plant 2: WT Plant 3: WT Plant 4: Mutant Plant 5: WT Plant 6: WT Plant 7: WT Plant 8: -undetermined- Plant 9: Mutant Plant 10: WT Plant 11: Mutant Plant 12: -undetermined-

5 Gene 1 mutant plant phenotype
Why no phenotype for 2nd gene?

6 Second gene plants genotype

7 Salk vs. my results Gene anatomy according to Salk online database Gene anatomy according to my lab results

8 When and where are the genes expressed?
Second Gene First Gene

9 Promoter region 1 What were the sequence results? 1-kb Ladder Clone #1

10 Promoter region gene 2 What were the sequence results?

11 Conclusion Gene 1 -found mutant -no visible phenotype -Promoter cloning obsolete Gene 2 -no found mutant -phenotype N/A -Promoter cloning has mutations


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