the manipulation of living organisms for human use Chapter 13

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the manipulation of living organisms for human use Chapter 13 Genetic Engineering the manipulation of living organisms for human use Chapter 13

Breeding strategies Selective breeding: only certain individuals with certain traits to reproduce Inbreeding: crosses individuals with similar traits Hybridization crosses individuals with different traits; tend to by hardier and healthier

Manipulating DNA Genetic engineering: making change in the DNA code of an organism Restriction enzymes: cut the DNA at specific sites by recognizing certain DNA sequences produces more manageable pieces of DNA to separate and analyze Gel electrophoresis: separates DNA fragments through a gel using electricity

Gel Electrophoresis

Using DNA Recombinant DNA: the manipulation of specific genes in the cells of living organisms causing them to produce new or unusual substances Polymerase Chain Reaction (PCR): uses DNA polymerase to create thousands of copies of a gene

DNA fingerprinting method of DNA analysis which identifies individuals by examining DNA fragments uses portions of DNA which do not code for proteins these sequences are unique to each individual, just like fingerprints Steps sample of DNA is cut using restriction enzymes fragments are separated using electrophoresis to create a pattern of bands

DNA Transformation An organism takes up DNA from another organism Steps Map the chromosomes of an organism Locate the specific gene of interest on the chromosome Cut the DNA with restriction enzymes Isolate and produce many copies of that gene (PCR) Insert gene into a plasmid then into the new organism’s DNA and regulate the expression

DNA Transformation

Application of Gen. Eng. Transgenic Organisms Contain DNA from another species Example: insulin-producing bacteria, glow in the dark fish 52% of soybeans, 25% of corn are genetically modified

Application of Gen. Eng. Cloning Clone: member of a population of genetically identical cells that are produced from a single cell In 1997, Ian Wilmut presented “Dolly”, the first cloned sheep

Steps of Cloning

Human Genome Project An ongoing effort to analyze the human DNA sequence Started in 1990, completed in 2000 Different scientists studied random fragments of DNA then combined their data Humans have approximately 35,000 genes