Invest. Ophthalmol. Vis. Sci ;49(10): doi: /iovs Figure Legend:

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Invest. Ophthalmol. Vis. Sci ;49(10): doi: /iovs Figure Legend:

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Invest. Ophthalmol. Vis. Sci ;49(10): doi: /iovs Figure Legend:

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Presentation transcript:

From: Mechanisms of Murine Lacrimal Gland Repair after Experimentally Induced Inflammation Invest. Ophthalmol. Vis. Sci.. 2008;49(10):4399-4406. doi:10.1167/iovs.08-1730 Figure Legend: The BMP7 pathway is activated during the repair phase. (A) Lacrimal gland homogenates prepared from control (C) and IL-1–treated animals were processed for Western blotting using an antibody against phosphorylated Smad1/5/8 or against β-actin (to control for gel loading). Data in the plot are mean ± SEM from three independent experiments. *Statistically significant difference from control. (B) Lacrimal glands removed from saline- and IL-1–treated animals were processed for immunofluorescence studies using the antibody against phosphorylated Smad1/5/8. (C) Lacrimal glands removed from IL-1–treated (3-day) animals were processed for double-immunofluorescence studies using anti-nestin (green) or anti-phosphorylated Smad1/5/8 (red). Nuclei were counterstained with DAPI (blue). Most of the nestin-positive cells were also positive for phosphorylated Smad1/5/8 (inset). Scale bar: (B, C) 50 μm. Date of download: 9/18/2016 The Association for Research in Vision and Ophthalmology Copyright © 2016. All rights reserved.