Photosynthesis vs. Respiration : A Comparison Mark Adame St. Mark’s School of Texas Dallas, Texas
Objectives For students to understand the general pathways of photosynthesis and cellular respiration For students to be able to compare & contrast the biochemical pathways of photosynthesis For students to be able to compare & contrast the structures involved in both pathways
The Transfer of Energy in Living Systems
Organelle Structure Mitochondrion –Outer phospholipid bilayer membrane surrounding an intermembrane space which surrounds a highly folded (cristae) inner membrane –Matrix –DNA, RNA, ribosomes Chloroplast –A double outer phospholipid bilayer membrane that houses a fluid-filled compartment called the stroma –Inner membrane system comprised of discs called thylakoids –DNA, RNA, ribosomes
Thylkoids Grana Stroma Double membrane The Chloroplast
Intermembrane space Inner membrane (cristae) Matrix The Mitochondrion
Organelle Function Mitochondrion –To generate ATP from chemical energy stored in organic molecules Chloroplast –To generate carbohydrates from light energy
An Overview of Cellular Respiration
An Overview of Photosynthesis
Overview of Cellular Respiration & Photosynthesis Glycolysis “Prep-step” Krebs (Citric Acid) Cycle Electron transport chain (ETC or ETS) Light-Dependent Reactions (Cyclic & non-cyclic) drive the electron transport chain Light-Independent Reactions (Calvin Cycle)
Some Biochemical Differences Photosynthesis requires light & only occurs in photoautotrophs Cellular respiration occurs in ALL eukaryotic organisms; both heterotrophs AND photoautotrophs (how else would photoautotrophs break down their sugars?)
Reactants & Products Reactants: –Photosynthesis Water & Oxygen Requires light energy & ATP –Cellular Respiration Organic molecules Products –Photosynthesis Sugars which lead to the production of organic molecules –Cellular Respiration Water & Oxygen Creates ATP for use by the cell
Remember This, Backwards & Forwards!!!
REDOX Reactions –“Have electrons, hydrogen ions will travel” H + e - 4 e H + “Who” gets the 4 e H + ?
Reactants & Products Photosynthesis Cellular Respiration “Who” gets “who's” e - + H + ?
Electron Flow Cellular Respiration –Glucose (cytoplasm) –Pyruvate & NADH –Acetyl-CoA (Matrix) –NADH & FADH 2 (Matrix) –Electron Transport Chain (inner membrane) –O 2 H 2 O (Matrix) Photosynthesis –H 2 O & photolysis –Photosystem II –Electron Transport Chain (Thylakoid membrane) –Photosystem I –NADPH (Stroma) –CO 2 (Stroma) –Glucose (starch & vacuole)
Building up & Breaking Down
Energy Pathways The products of first set of reactions in photosynthesis (light reactions) are used to generate carbohydrates (energy gained by reactants) The products of first set of reactions in cellular respiration are used to generate energy to create ATP (energy lost from reactants) Photosynthesis is an anabolic pathway Cellular respiration is a catabolic pathway
ATP & Energy For each molecule of glucose broken down in cellular respiration, up to molecules of ATP may be generated For each molecule of glucose generated by photosynthesis 18 ATP are used. –These ATP, however, are generated from light energy
Some Similarities
Chemiosmosis & ETC Electron source Electron Carriers (NADPH, NADH) Electron Transport Chain (Cytochrome) Electron sink Proton gradient across an inner membrane Proton motive force ATP Synthase & ATP Production
ATP Generation
NADPH vs. NADH Both cofactors that transport electrons –NADPH aka nicotinamide adenine dinucleotide phosphate –NADH aka Nicotinamide adenine dinucleotide
Additional Differnces/Similarities? List any additional differnces and/or similarities below
Laboratory 4 Plant Pigments & Photosynthesis Pigment properties Chromatography Light reactions & DPIP reduction –6CO 2 + 6H 2 O --sun--> C 6 H 12 O 6 + 6O 2 Spectrophotometers
Chromatography –Pigment properties –Solvent –Hydrogen-bonding –R f Values
Light Reactions DPIP (2, 6-dicholorophenol -indophenol) replaces NADPH Spectrophotmeter & in % transmittance
What is expected from the results
Laboratory 5 Cellular Respiration Aerobic Respiration Respirometers & Gas Law C 6 H 12 O 6 + 6O > 6CO 2 + 6H 2 O kcal/mole (or ~34-36 ATP) Germination of seeds
PV = nRT The Concept: Using the gas laws, cellular respiration can be measured in a closed container.
2KOH + CO 2 K 2 CO 3 + H 2 O n is decreasing for CO 2 & increasing for O 2 C 6 H 12 O 6 + 6O 2 6CO 2 + 6H 2 O
Free Response ENERGY QUESTION 1995: –Energy transfer occurs in all cellular activities. For 3 of the following 5 processes involving energy transfer, explain how each functions in the cell and give an example. –Explain how ATP is involved in each example you choose. –1. Cellular movement –2. Active transport –3. Synthesis of molecules –4. Chemiosmosis –5. Fermentation
Free Response CELLULAR RESPIRATION QUESTION 1982: –Describe the similarities and differences between the biochemical pathways of aerobic respiration and photosynthesis in eukaryotic cells. Include in your discussion the major reactions, the end products, and energy transfers.
Various Links Resources/Metabolic_Pathways.html otosynthesis_1.html