Develop a Bandwidth Detection System to Monitor Metabolic Activity of Single Cells  Jerry Wilmink and Jonathon Wells  Advisor: Franz Baudenbacher  BME 273, Group 9

Project Inception?  BioMEMS Device  Funded by DARPA  Cell based sensing technology  Monitor Cell Metabolism  Subnanoliter volumes

BioMEMS  Fabricated on Silicon Chip Peristaltic Pumps Fluidics Layer  Interdigitated Array electrodes

BioMEMS  Combine electrodes with flourescence probes  Quantify and Classify the impact of various toxins on cell physiology  CBW Agents to develop signatures Expected Acidification Rate of a Single Cell in a 0.1 nL Volume

Project Overview  Step 1: Design the hardware for a 256 channel data acquisition system to read out the Hamamatsu photodiode array

Project Overview  Step 2: Design Labview program to control the data acquisition system and provide control signals for the valves driving the bioMEMS device

Project Overview  Step 3: Design Hardware to interface the photodiode array with an inverted microscope and the bioMEMS devices

Project Overview  Step 4: Devise a calibration protocol for the photodiode array bandwidth of dye

Project Overview  Step 5: Record transmembrane potential of single cardiac myocytes with flourescent probes and record from electrochemical sensors

Progress  Research Darpa Proposal Journals, Internet etc  Began Step 1: Install Labview Install NI data acquisition board Soder individual channel to corresponding photo diode Apply current to bioMEM to make sure it worked

Future Plans  Begin Step 2: Design Labview program That enables specific channel identification That is read in from specific photodiodes Work with potentiostat readout and manipulate and quantify results