1. A Bandwidth Detection System to Monitor Single Cells
Jerry Wilmink and Jonathon Wells
Advisor: Franz Baudenbacher
BME 273, Group 9

2. Presentation Overview
DARPA and BioMEMS
Our Project Design
Overview of Project
Progress
Future Plans

3. DARPA at Vanderbilt BioMEMS Device Funded by DARPA
Cell based sensing technology
Monitor Cell Metabolism
Subnanoliter volumes
Bio MicroElectroMechanical Systems
Defense Advanced Research Projects Agency

4. BioMEMS Fabricated on Silicon Chip Interdigitated Array electrodes
100micrometers
Fabricated on Silicon Chip
Peristaltic Pumps
Fluidics Layer
Interdigitated Array electrodes
Interdigitated Array electrodes- allow monitior cells metabolic activity by measuring the uptake of oxygen and glucose
.25nL

5. Array Output of Intensity of Signal
Project Design
Light
BioMEM Device, single cell w/dye
Microscope
DAQ Board
Amplifier
Labview
Array Output of Intensity of Signal
Voltage
Photodiode Array

6. Project Overview Step 1:
Design the hardware for a 256 channel data acquisition system to read out the Hamamatsu photodiode array
Picture o f data acqusiiton board

7. Project Overview Step 2: Picture of LABVIEW
Design Labview program to control the data acquisition system and provide control signals for the valves driving the bioMEMS device
Picture of LABVIEW

8. Project Overview Step 3: Picture of LABVIEW
Design Hardware to interface the photodiode array with an inverted microscope and the bioMEMS devices
Picture of LABVIEW

9. Project Overview Step 4: Picture of LABVIEW
Devise a calibration protocol for the photodiode array
bandwidth of dye
Picture of LABVIEW

10. Project Overview Step 5:
Record transmembrane potential of single cardiac myocytes with flourescent probes and record from electrochemical sensors
Flourescent probe= the measurement from the cell
Electrochemical sensor= interdigitated array

11. Progress Research Darpa Proposal Journals, Internet etc
Completed Steps 1, 2
Started Step 3:
Modifications of Labview Program
Collects in 2D array
Need 3D Array (Data Organization)
Testing to make sure that the gain is linear across all of the channels

12. 3D Array

13. Future Plans Finish Step 3: Modify Labview program
Organize data in 3D array
Begin taking measurements