1. Design Team 8: Fluorescent Detection using Optical Fibers with Cardiac Myocytes Team Members: Paul Clark Martin Garcia Chris Gorga John Ling III Giordano Lo Regio Advisors/Assistants: Dr. Franz Baudenbacher Raghav Venkat Tobias Meyer

2. Introduction Why BioMEMS? – Create experiments on Micro scale Why BioMEMS? – Create experiments on Micro scale Importance of calcium detection in cardiac myocytes Importance of calcium detection in cardiac myocytes –The amount of calcium in a cell can be used to quantify the force (length and strength) of the contraction of a cardiac cell Use of fluorescence and optical fibers to determine calcium concentrations Use of fluorescence and optical fibers to determine calcium concentrations –Best way to measure calcium concentrations on a micro scale level

3. Marketable Motivation Customer/User Customer/User –Researchers –Decreased amount of dye needed Business Business –Decreased amount of funding for research Society Society –Improved diagnosis/treatment based on findings

4. Completed Work A three channel, photographic mask has been developed A three channel, photographic mask has been developed First step of clean room certification achieved First step of clean room certification achieved A LabView module is completed to record the magnitude of the fluorescence signal A LabView module is completed to record the magnitude of the fluorescence signal Strategy in place Strategy in place

5. Slide Design Optical Fibers

6. Current Work Fabricating prototype devices while waiting for the completion of clean room certification Fabricating prototype devices while waiting for the completion of clean room certification Perfecting the LabView module Perfecting the LabView module Casting PDMS devices with different size optical fibers Casting PDMS devices with different size optical fibers –Therefore the minimum dye concentration necessary for detection can be found

7. Future Work – improve limit of detection Once the minimum dye concentration has been found… Once the minimum dye concentration has been found… –Chopper –Expect signal with narrow bandwidth Lock-in amplifier to remove noise Lock-in amplifier to remove noise –Combines DC and AC amplifiers with a series of low and high pass filters

8. Future Work – cell incorporation Capture cells in device Capture cells in device –Fibronectin will be used to adhere cells Use a pH sensitive dye Use a pH sensitive dye –Find correlation with Ca ++ concentration and acidification rates –Carboxylated Flouresein

9. Timeline Week of January 22 Week of January 22 –Obtain clean room certification –Begin casting optical fibers in PDMS Week of January 29 Week of January 29 –Prepare various flourescein concentrations and begin data acquisition with all 3 optical fiber diameters Week of February 5 Week of February 5 –Adapt device to incorporate cells –Test device