2. To-Do Monday, 22 February 2016 Orders Safety Lab Upkeep Protocols Oracle Calendar Lab Logo

3. 2 Orders http://hpgl.umd.edu/mediawiki-1.9.3/index.php/List All items that you would like purchased need to be entered into the lab wiki You may temporarily write items on the white boards, but they must eventually go in the wiki for me to place the order To prevent confusion, forgotten orders, redundancy, and other issues, only items entered into the wiki will be considered for purchase Order items in advance, before we run out! It may take me several days to place the order, several more days for it to process and ship, and it may still be backordered You need to include the item number and vendor if you are reordering an item If it is a new item, please research it and post the best option or several options for me to review If necessary, post several options and ask me to check prices Always include item numbers A list of reagents is located next to the white board in the lab and under the shelves of chemicals – refrigerated/frozen items that came from TIGR are not on this list – check this list before you ask me if we have something

4. 3 Orders To Order Items that need to be ordered Put the newest entry at the top of the list Follow the format shown at the top of the page Tentative Items we are considering for purchase, but don't need immediately Reminders, not order requests Ordered I move items here after the order has been placed Will be updated with the order date, the expected ship date, backorder information, etc. so check for updates frequently

5. 4 Safety First Aid Kit – Under white board by RA room door Let me know if you use/remove items from the kit – it has a minimum stock requirement Fire Extinguisher – Under white board by RA room door Fire Alarm & Closest Exit – Stairwell to the left, down the hall Shower – Across from TC room, next to the lab door Eye Wash – Next to Sink – Run periodically (once every week or two) Chemical and Biological Spill Clean-Up Kits – We should assemble these ourselves MSDS – In RA Room File Cabinet - “Equipment Manuals” Drawer

6. 5 Safety Safety Glasses, Goggles, and Face Shield Use when boiling solutions on the bench, or working with caustic chemicals or irritants Use when working with the UV transilluminator w/o the shield (transferring or cutting gels w/ UV on) Ask me about their locations Hearing Protection Must be worn when working with the sonicator 3 pairs of earmuffs have been ordered Broken Glass Containers Empty the small containers into the large box next to the big centrifuge and reuse the small boxes if possible Chemical Waste Containers? Wear Lab Coats!

7. 6 Lab Upkeep Drying Rack Place washed glassware/plasticware on the drying rack, not on the bench surrounding the sink When it is dry, return the glassware/plasticware you washed to the cabinets Don't leave stirbars, forceps, or other small items near the sink – dry them with a papertowel and put them away Sinks Clean up after yourself and wipe down the sink at the end of the day to prevent staining, sitting water, etc. Don't leave items in the sink – they can break or go down the drain DO NOT WASH GEL SOLUTIONS DOWN THE SINK Let larger volumes of gel solutions solidify (in the glassware, gel tray, etc...) and appropriately dispose of as solid waste (trash or EtBr waste)‏ If it is a very small amount, ensure it is a liquid and dilute with a large volume of water before washing it down the sink so it doesn't resolidify

8. 7 Lab Upkeep Common Equipment Areas Proposal 1 – Everyone cleans up after themselves and returns the equipment to a state immediately usable by the next person Proposal 2 – Each person is responsible for keeping the common equipment areas in their bench row clean and organized – I will maintain equipment not on lab bench rows, such as the balances and pH meter Of course, tell me of any equipment malfunctions or problems Hoods Keep them clean and sterile Remember to turn them off when not in use There is a dust pan and broom by the broken glass container between the refrigerator and centrifuge Trash needs to be put outside the lab at least weekly Perhaps set the bags in the hall so we don't lose our trashcans

9. 8 Protocols http://hpgl.umd.edu/mediawiki- 1.9.3/index.php/Protocols http://hpgl.umd.edu/mediawiki- 1.9.3/index.php/Protocols No one has added any protocols to the wiki since I have on September 5 th Please write up your more permanent protocols using the format of an existing protocol as a template It probably isn't worth generating a protocol for a method you are still trouble-shooting, but it would be a start You may create separate pages or sections within Protocols to better organize them

10. 9 Oracle Calendar http://calendar.umd.edu/ Schedule and Modify Lab Meetings Post Important Dates and Seminars Automatic E-mail Notifications Accessible Anywhere Need UIDs to Set Up Accounts Log In Using Directory ID and password

11. 10 Miscellaneous Fyrite Kit Try using Brenda’s fyrite kit – Camila If we like it, we can share Brenda’s and purchase the fluids or we can buy our own kit Biomek Robot http://hpgl.umd.edu/mediawiki-1.9.3/index.php/Biomek_FX_Robot Order extra supply containers for degassed pump water Preliminary Projects Automate PCR using an entire plate of primers Automate PCR by picking primer pairs from plates of primers Automate BP Clonase II Reaction to transform the PCR products into the Entry Vectors

12. Lab Logo Lab Name Host-Pathogen Genomics Laboratory Can we be more unique? T. brucei Protein Interactome T. cruzi MASP

14. TrailBlazer Tour! After the lab meeting?