1. Fast and Precise! Introducing the NucleoCounter®NC-250™ How healthy are your cells? It’s VITAL to know!

2. Fast and Precise! Precise and objective No manual adjustments, just press RUN High speed cell count 8 samples in less than 3 minutes! Counting & Analytical Assays Cell health determination via fast apoptosis assay Low running costs Reduce your running costs significantly Calibration and maintenance free Instantly ready NC-250™ Highlights

3. Fast and Precise! One-Step Viability and Cell Count Load and press Run Two-Step Viability and Cell Count Micro-Carriers – extremely aggregated cells Two-step Cell Cycle New, innovative 5 min. Cell Cycle Assay Cell Vitality Cell health determination via the fastest apoptosis assay What if you knew your cells’ health before they were dying? Pre-defined assays (Just press Run!)

4. Fast and Precise! NC-Slide™ A2 and A8 NC-Slide A2™ NC-Slide A8™ Fast and Precise! Only a small sample volume needed! 10 µl sample Low volume Fast measurement 30 µl sample Better statistics

5. Fast and Precise! Fast and easy to use... Measure up to 8 viability and cell counts in less than 3 minutes Maintenance, calibration and service free Simply mix your cells with dye, load and press RUN!

6. Fast and Precise! Fast results: Up to 8 viability & cell counts in less than 3 minutes! Results  Total count  Viability  Cell diameter  Cell Aggregates in %  Export to Excel Validation  21 CFR Part 11 ready  IQ/OQ/PQ

7. Fast and Precise! Superior Visual Inspection Objective results! Visual control

8. Fast and Precise! Superior Data Presentation Browse data from prior samples Easy selection of protocols Wizard for user defined protocols Easy access to application notes Visual inspection of counted cells Easy export of data

9. Fast and Precise! Protocol Adaption Wizard User friendly guide for protocol adaption. Create new protocols for specialized applications.

10. Fast and Precise! Superior precision Cell Conc. (cells/ml) No. of cells Counted in A2 Theoretical SD (cells) Theoretical CV 5 x 10 4 560 24 4,2% 1 x 10 5 1120333,0% 5 x 10 5 5600 751,3% 1 x 10 6 112001060,9 % 5 x 10 6 56000237 0,4% 1 x 10 7 1120003350,3% Cell Conc. (cells/ml) No. of cells Counted in A8 Theoretical SD (cells) Theoretical CV 5 x 10 4 120 11 9,1% 1 x 10 5 240156,5% 5 x 10 5 1200 352,9% 1 x 10 6 2400492,0 % 5 x 10 6 12000110 0,9% 1 x 10 7 240001550,6%

11. Fast and Precise! Two-Step Cell Cycle Assay Fast and easy cell cycle assay Directly from T-Flasks or wells (no detachment step) No ethanol fixing No RNase treatment required Standardized Results Fast, high precision Cell Cycle Assay

12. Fast and Precise! Vitality Assay (Apoptosis/Cell Health) Fast and easy Apoptosis/Cell Health assay For easy detection of changes in levels of reduced thiols In 4 minutes, get 8 Apoptosis measurements. Find out how healthy your cells are…

13. Fast and Precise! Specifications Loading volume:30 µl for NC-Slides A2™, 10 µl for NC-Slides A8™ Measurement volume:Viability and Cell Count Assay: 11.2 µl for NC-Slides A2™ and 2.4 µl for NC-Slides A8™. Analysis time:Viability and Cell Count Assay: 8 samples in 3 minutes for NC-Slide A8™. 2 samples in 2 minutes for NC-Slide A2™. Measurement range:Viability and Cell Count Assay: 5 x 10 3 to 1 x 10 7 cells/ml Optimal range:Viability and Cell Count Assay: 5 x 10 4 to 5 x 10 6 cells/ml Size:19 x 29 x 32 cm (W x H x D), weight 9 kg Software:NucleoView™ NC-250 computer software for documentation and presentation

14. Fast and Precise! Cell Count (2-step Viability) 1-step Viability and Cell Count Vitality Cell Cycle NC-100, NC-200, NC-250, NC-3000 NC-3000 Apoptosis GFP Biomarkers NC-200, NC-250, NC-3000 NC-250, NC-3000 NC-3000 Scalability & flexibility Function needs Product range

15. Fast and Precise! Fast and Precise Cell Counter The NucleoCounter® NC-250™ The right instrument if precision, speed and cell health determination are important factors to you. 990-0252 ver. 01.2013 Precise and objective No manual adjustments, just press RUN High speed cell count 8 samples in less than 3 minutes! Counting & Analytical Assays Cell health determination via fast apoptosis assay Low running costs Reduce your running costs significantly Calibration and maintenance free Always ready in seconds