5MxPro 4.1 General functionality Plate Setup:No well names in standard view in previous versions. Only in fullscreen plate viewShow Well Names button
6Setting Well NamesWell names are accessed under the Setup Screen for each experiment, just as this would be set in a single experiment.
7MxPro 4.1 General functionality Thermo Profile Setup:Custom setup as in old Mx softwarePredefined one click setup using Standard Design:Add RT step, normal or fast or 3-step cycling protocol, dissociation curve Estimated run time shown
8MxPro 4.1 General functionality Default Sets options:Apply to new experimentsApply to opened experimentsMxPro 3.x preferencesMxPro 4.1 preferences
9MxPro 4.1 General functionality Analysis section:Lock thresholds: Threshold is fixed to current value. Selection of different part of plate doesn‘t change threshold when locked.Customizable ToolbarLabel graph according to replicate/well ID or well nameMxPro 3.x: Amplification plotsMxPro 4.1: Amplification plots
10Improved Plate Setup functionality: Assay name/gene target identification so one dye can have multiple assay/gene namesNew Excel template for plate setup importWell names and quantities automatically across all replicate wells from a single entryDye editing in plate setupAdd right mouse-click capability for easier access to well names, analysis options, legend, and other related functions
11Plate Setup – Importing, Well Name/ID, Assay Names
12Add Dye Editing from Plate Setup Goal: Easy dropdown provides access to dye list without having to go to Optics Configuration menu.StandaloneInstrument-Connected
13Ease-of-Use: Added Functionality Right Mouse-Click Functionality
14Right Mouse Click Functionality Plate SetupThermal Profile SetupRaw Data Plots
16Comparative Quantification using MX Pro Software
17Enhancements in MxPro Software Improved Comparative Quantification functionality:Comparative Quantification analysis for multiple assays/genes simultaneouslyAddition of Relative Quantity Chart for log fold change which is the most common type of published data chart for this type of dataAdd error bars based on replicate variability to Relative Quantity ChartView Amplification Plots and Standard Curve on same screen
18Comparative Quantification in Mx Software Given two samples one gene and one normalizer: What is the difference in gene expression?Control (Calibrator)Sample 1 to x#Gene of InterestCtGOI RatioNormalizerCorrection FactorCtNormalized sample GOI fold change compared to control
19Comparative Quantification in MxPro Software Given two samples, multiple genes, and a single normalizer: What is the difference in gene expression?Control (Calibrator)Sample 1 to x#Gene of Interest: ACtGOI Ratio for Gene AGene of Interest: BCtGOI Ratio for Gene BGene of Interest: CCtGOI Ratio for Gene CNormalizerSingle Correction FactorCtNormalized sample GOI fold change for Gene A, B, and C compared to control
20Comparative Quantitation Plate SetupAssign assay names for analysis of multiple genes to a single normalizer (or unique normalizers) simultaneously.
21Setting Assay NamesAssignment of Assay Names is accessed under the Setup Screen for each experiment, just as this would be set in single experiment mode.
22Comparative Quantification In a normal single plate analysis, the software associates normalizer wells to the corresponding GOI wells from the same sample using Association Symbols.In a Project these are associated together differently across experiments, instead using Well Names.Plate 1Plate 2
23Creating Associations Assignment of Associations for comparative quantification is accessed under the Setup Screen for each experiment, just as this would be set in single experiment mode.
26Comparative Quantitation: Multiple Gene Targets Fold gene expression changes for six genes across two treatments with replicate error represented.
27Absolute Quantification If you are comparing unknowns on one plate to a Standard curve run on a separate plate, the software will calculate quantities for your Unknowns based on that curve as long as both sets of wells have the same assay name.
28Absolute Quantification of Unknowns Assignment of Assay names for absolute quantification must be the same and is accessed under the Setup Screen for each experiment.
29Show Amp Plots and Standard Curve on One Screen View placement of threshold in Amplification Plots as well as the impact to Standard Curve simultaneously.
30Customize Plot/Legend: Add Amp Plot Color Coding Based on Dye Show groups by dye/assay color or by well name
32Enhancements in MxPro Software Improved Text Report functionality:Addition of new columnsAnalysis properties displayed at top of text reportAddition of text report sorting by different columnsGenerate customized consolidated data reportsAuto generate Excel charts when exporting data
33Text Report: Sort Text Report Columns Based on Plate Column Group data for reporting based on potential biologist plate setup and further customize data reports from software.
34Text Report: Place Dye Analysis Parameters at Top of Text Report Reduce redundancy in text report (dye/assay specific information does not need to appear in every row of main report) and generate a cleaner report format.
35Consolidated Data Reports Provide one screen data viewing and printing of all desired screens/reports.
36Export Results in Multiple Formats Text ReportAny screen shotExcel spreadsheetPowerPoint presentationAll of the data generated as well as the results of the analysis can be exported in multiple formats. Numerical results can be exported as text files or directly opened in Microsoft Excel. All plots and graphs can also be exported into Excel or directly into PowerPoint where a new presentation can be generated with a single click of the mouse.
37Generate Excel Charts when Exporting Charts Provide modifiable Excel charts for biologist customization and ease of use.