1. GC chromatograph of OC and SP pesticides mixture with method used in present study Sr. no. Name Retention time (m) Sr. no. Name Retention time (m) 1α- HCH12.7814β-endosulfan25.48 2β- HCH13.8315p,p’ DDD25.80 3γ –HCH13.9817 Endosulfan sulphate 27.16 4δ- HCH15.2418p,p’ DDT27.36 5Heptachlor17.1819Cyhalothrin31.51 7Aldrin19.0420Permethrin33.48 8Fipronil19.4121Cyfluthrin34.47 9Butachlor19.6322Cypermethrin35.12 11p,p’ DDE22.3723Fenvalerate37.38 12o,p’ DDD24.2124Deltamethrin38.90 13Endrin24.85

2. Organophosphorus pesticides Peak no. Name of pesticideRetention time (min.) 1Monocrotofos6.067 2Dimethoate6.717 3Parathion methyl8.381 4Malathion8.837 5Chlorpyrifos9.215 6Profenophos10.973 7Ethion11.209 8Triazophos12.217 10Phosalone16.307 GC chromatograph of OP pesticides mixture with method used in present study.

3. GroupRetention time window (min.)m/z (compound) 111.80-12.30192, 164, 127 (Monocrotofos) 212.40-13.10219, 183, 181 (α-HCH), 125, 87 (Dimethoate) 313.30-14.30219, 183, 181 (β-HCH, γ-HCH) 415.00-15.70219, 183, 181 (δ-HCH), 263, 233, 125 (Parathion methyl) 516.20-16.50173, 158 (Malathion) 616.60-17.00314, 286, 97 (Chlorpyrifos) 717.10-17.50337, 272, 100 (Heptachlor) 818.80-19.20277, 263, 261 (Aldrin) 919.50-20.30251, 250, 139 (Butachlor), 339, 139 (Profenophos) 1021.30-22.00231, 153 (Ethion) 1122.20-23.20318, 246, 241 (p,p’ DDE) 1223.60-24.60318, 237, 235 (o,p’ DDD), 81, 263 (Endrin) 1325.20-26.10241, 237, 195 (β-endosulfan), 235, 178, 165 (p,p’ DDD) 1426.90-27.50420, 387, 272 (Endosulfan sulphate), 237, 235, 212 (p,p’ DDT) 1531.30-31.90449, 197, 181 (Cyhalothrin) 1634.30-35.60226, 206 (Permethrin), 163 (Cyfluthrin), 181, 163 (Cypermethrin) 1737.00-37.80419, 167, 125 (Fenvalerate) 1838.70-39.10253, 182 (Deltamethrin) Retention time windows and mass to charge ratios (m/z) used for selected ion monitoring in GCMS m/z- mass to charge ratio

4. GC and GCMS chromatographs of pesticides residues detected in breast milk samples

5. Figure: GC chromatograms of γ-HCH (A) detected in milk sample, GCMS (B), ion spectrum of sample (C) and standard (D) m/z (C) 2.55.07.510.012.515.0 0.0 1.0 2.0 3.0 4.0 uVuV (x10,000) E:\GC DATA/ γ-HCH Sample Chromatogram 1//15.335 γ-HCH 13.5513.6513.7513.8513.9514.0514.1514.25 0.25 0.75 1.25 1.75 2.25 2.50 (x1,000) 181.00 (2.43) 183.00 (1.98) 219.00 (1.00) TIC 1/Gamma-HCH/13.999 (B)(A) (D) Voltage Time in minutes Voltage

6. 5.015.025.035.045.055.060.0min 0.00 1.00 2.00 3.00 4.00 uV(x100,000) E:\GC DATA/pp DDE Chromatogram 1/pp DDE/31.484 22.222.322.422.522.622.722.822.923.023.1 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 (x1,000) 248.00 (1.77) 246.00 (1.20) 318.00 (4.83) TIC 1/pp DDE/22.762 180190200210220230240250260270280290300310 0.0 2.5 5.0 (x1,000) 246 175 318 231 180190200210220230240250260270280290300310 0.0 2.5 5.0 (x1,000) 246 318 176 210 281 245 Voltage Time in minutes m/z (D) (A) (C) (B) Figure: GC chromatograms of pp DDE (A) detected in milk sample, GCMS (B), ion spectrum of sample (C) and standard (D)

7. 25.125.325.525.725.926.126.2 0.25 0.75 1.25 1.75 2.25 2.75 (x10,000) 165.00 (2.19) 235.00 (6.38) 237.00 (6.67) TIC 1/pp DDD/25.671 165.0170.0175.0180.0185.0190.0195.0200.0205.0210.0215.0220.0225.0230.0235.0240.0 0.0 2.5 5.0 (x1,000) 165 207 241 235 195 237 182 Voltage m/z 1/pp DDD/33.435 5.010.015.020.025.030.035.040.0 min -0.25 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 uV (x100,000) E:\GC DATA\pp DDD Chromatogram Voltage Time in minutes (A) (B) (C) (D) Figure: GC chromatograms of pp DDD (A) detected in milk sample, GCMS (B), ion spectrum of sample (C) and standard (D)