1. Engineering Biology Chee 450 Prof. Ron Neufeld Dupuis 230, 533-2785 neufeld@chee.queensu.ca

2. Acknowledgments Many images in this course originate from the web. The source may be determined by placing your cursor over the image. In a few cases, images were scanned from textbooks or literature journals. The source of any image may be obtained by contacting neufeld@chee.queensu.ca.neufeld@chee.queensu.ca A few of the images actually belong to the instructor. Instructor before he busted his electric shaver

3. Office hours and location 7:48 am to 5:39 pm, Monday to Friday if I am there e-mail any time – response usually same day office over front “smoking” entrance of Dupuis Hall lab office Biosciences 1443

4. Course web page reminders course outlines (and notes) assignments (and solutions) test and exam dates

5. Course objectives Biochemical Engineering is the application of Chemical Engineering principles and approaches to biologically-based systems and processes Biological Engineering will examine the underlying biological and biochemical principles

6. Course text Zen and the Art of Motorcycle Maintenance, by Ronald DiSanto

7. Reference material Shuler and Kargi, Bioprocess Engineering: Basic Concepts Blanch and Clark, Biochemical Engineering Bailey and Ollis, Biochemical Engineering Fundamentals

8. More reference material Palsson, Hubbell, Plonsey and Bronzino, Tissue Engineering Lanza, Langer and Vacanti, Principles of Tissue Engineering Prescott, Harley and Klein, Microbiology (section on Industrial Microbiology)

9. Search for Stem Cells on: http://www.eurekalert.org/ EurekAlert is run by the American Association for the Advancement of Sciences. http://scitechdaily.com/ Range of news articles, features, and commentaries about science and technology http://www.sciam.com/index.cfm Scientific American website http://www.reuters.com/news.jhtml?type=science Reuters news site for stories about science. Search, then select "News and Pictures."

10. Course evaluation mid-term test: 25% assignments: 20% design project/report: 15% final exam: 40% contributions of cookies and muffins to class: according to level of class participation

11. History and overview of the products of Biochemical Engineering Citric acid Antibiotics Brewing/Bioethanol Amino acids Monoclonal antibodies Therapeutic proteins Farmaceuticals Site directed mutagenesis

12. Principles of Cell Culture Properties and structure of the cell Nomenclature Bacteriophage Growth behavior Determining cell mass Chemical composition of cells

13. Tissue engineering Tissue culture with mammalian and plant cells Products and medical applications Large-scale mammalian cell culture systems

14. Metabolism and product formation Metabolic pathways – catabolic/anabolic Biosynthesis Stoichiometry and energetics Control and regulation Industrial strain improvement Screening for new metabolites Molecular genetics and control

15. Enzymes and enzyme kinetics (versus Monod growth kinetics) Naming and classification Applications – purification of racemic mixtures of amino acids Reaction kinetics (Michaelis-Menten) Inhibition Batch and continuous reactor kinetics

16. Immobilized biocatalysts Immobilization methods Immobilized enzyme/cell kinetics Mass transfer resistance, effectiveness factors

17. Product separation and purification Downstream process scheme in protein purification

18. Design project: A downstream separation train will be examined in detail and each of you assigned one particular unit operation within the purification protocol for a detailed design. The design will be presented in class, followed by a written report.