1. Unique Microplates for
UVStar®
Unique Microplates for
the UV-Range

2. Pre-UVStar®-Period At my time, only quartz glass was
available, and it was
fragile
expensive Microplates
hard to clean for reuse
limited in throughput

3. Uniqueness of UVStar® Microplates with outstanding optical properties
Dedicated for OD reading in the UV-range
Proprietary, unique resin
Optical window down to 200 nm
Available in 96 and 384 well format

4. Prerequisites for UVStar®
Availability of a unique proprietary plastic
Material free of any UV-blocking additives
Production of high quality films (140 µm)
Lamination process free of glue and solvents
Adaptation of µClear®-process to UVStar®
UVStar_Rev. 1

5. µClear®-Technology Direct lamination technology
Process free of glue or solvents
µm films
Enables multi-mode detection
Worldwide Patents
Germany: DE
Europe: Pat. pending
USA: Pat. pending

6. Applications of UVStar®-Plates
Optical window down to 200 nm
Background: < nm
260 nm
Purity of 260/280 nm
Protein 280 nm

7. Absorbance of DNA vs. Protein

8. Clear Bottom Plates 1 mm bottom 140 µm bottom bonding of glass sheets
bonding of plastic films
2 component injection molding

9. Advantages of µClear®-Technology

10. From µClear® to UVStar®

11. Absorbance of UV-transparent Resins Greiner vs. Competitors

12. UV-Light Absorbance 384 well µClear® vs. UVStar®

13. Measurement of DNA-Conc. 96 vs. 384 Well UVStar®-Plates

14. Cell Growth (WST) in TC-treated 384 Well UVStar®-Plates

15. Growth of CHO-Cells in TC-treated 96 Well Plates

16. Homogeneity of 384 Well Plates

17. Performance of Background 384 Well UVStar® @ 260 nm

18. Drift and Background Greiner vs. Competitor

19. Drift and Background Greiner vs. Competitor
Mean 0,0524
Standard Deviation 0,002
Correlation of Variation 4 %
Mean 0,0498
Standard Deviation 0,001
Correlation of Variation 2 %

20. Pathlength & Lambert Beer’s Law I = Io ·e-k’ · c · x
Well pathlength [x]:
not defined
Cuvette pathlength [x]:
fixed

21. Correlation Pathlength vs. Absorption
@ 260 nm
Quartz Cuvette
[10 mm] = 0,08 OD
UVStar_Rev. 1

22. Chemical and Thermal Resistance
UVStar_Rev. 1

23. Benefits of UVStar®-Plates
Available in a 96 well and 384 well format
Extended optical window from 200 to 1000 nm
Background less than nm
Minimized variation from well to well
Ideal for quantifying DNA and total protein
No more need for expensive/fragile quartz plates

24. UVStar®
Made by