1. Lab 15 Goals and Objectives: Exercise 22: Mold Slide Culture Prepare slides as indicated on pg 165 except skip the 95% ethanol step (works fine without it) from your Rhizopus stolonifer and Penicillium notatum cultures Exercise 8: The Fungi Yeasts and Molds Observe your slides from Ex 22 for hyphae (septate or coenocytic) and asexual spore (conidiospore or sporangiospore) types Observe mold cultures on plates for spore color and colony morphology (provided by PA) Make wet mounts of yeast for observation, skip adding methylene blue (live yeast will not stain): Saccharomyces cervisiae (budding, no spores)(represents budding yeast) Candida albicans (budding, no spores) Candida albicans (hyphal form: incubated ~3-5hr in animal serum at 37°C) (represents dimorphic fungi) Quicktime movie: C. albicans budding → hyphal

2. Figure 8.1

3. Figure 8.2

4. Figure 8.6

5. Figure 8.5

6. Candida albicans Hyphal Growth & Pseudohyphal Growth PLAY

7. Slime Mold PLAY

8. Slime Mold on Long Island Physarum polycephalum Plasmodium spread in a single afternoon Next morning it was sporulating

9. Conidiospores ConidiophoreGerminating spore Sporangium Sporangiospores Coenocytic hyphae Septate hyphae Septa Rhizopus stolonifer Penicillium notatum MOLD FUNGI YEAST FUNGI DIMORPHIC FUNGI Saccharomyces cerevisiae Candida albicans Bud Hyphae Pseudohyphae Bud

10. Lab 15 Goals and Objectives: Exercise 22: Mold Slide Culture Prepare slides as indicated on pg 165 except skip the 95% ethanol step (works fine without it) from your Rhizopus stolonifer and Penicillium notatum cultures Exercise 8: The Fungi Yeasts and Molds Observe your slides from Ex 22 for hyphae (septate or coenocytic) and asexual spore (conidiospore or sporangiospore) types Observe mold cultures on plates for spore color and colony morphology (provided by PA) Make wet mounts of yeast for observation, skip adding methylene blue (live yeast will not stain): Saccharomyces cervisiae (budding, no spores)(represents budding yeast) Candida albicans (budding, no spores) Candida albicans (hyphal form: incubated ~3-5hr in animal serum at 37°C) (represents dimorphic fungi) Quicktime movie: C. albicans budding → hyphal