Presentation on theme: "Presented by: Emily Campbell PI: Dr. Stephen Giovannoni Mentor: Paul Carini Marine Microbiology Lab"— Presentation transcript:
Presented by: Emily Campbell PI: Dr. Stephen Giovannoni Mentor: Paul Carini Marine Microbiology Lab http://giovannonilab.oregonstate.edu
Discovered in 1990 by Stephen Giovannoni Most abundant heterotrophic organism in ocean surface waters world wide 1/3 of cells present in ocean surface water Role of SAR11 Dissolved organic carbon cycling SAR11 oxidizes carbon → Releases CO 2 Ocean lacks large quantities of key nutrients CO 2 Organic Carbon AutotrophsHeterotrophs
Most commonly found as phosphate (PO 4 ) An example of a nutrient found at low concentrations Below 5 nM Required for all living things Nucleotides (DNA and RNA), phospholipids (cell membranes) SAR11 thrives in low phosphate environments
Hypothesis: Each SAR11 isolate can utilize different phosphorus compounds with varying abilities. Prediction: If the isolate is able to utilize the given phosphorus-containing compound, we expect to observe maximum cell densities in excess of those cultures to which no phosphate has been added.
Verify results Free phosphorus in stocks Test positive results in triplicate What molecular pathways the compound could be used in What other necessities the phosphorus compound being transported could alleviate
Dr. Stephen Giovannoni Paul Carini HHMI Dr. Kevin Ahern