Presentation on theme: "LD50 of salt solution on Mung beans"— Presentation transcript:
1LD50 of salt solution on Mung beans Serial DilutionsLD50 of salt solution on Mung beans
2Cut piece of paper towel and insert into bag Get 2 x 3 inch jewelry bagsjewelry supply store. See product # on secondCut piece of paper towel and insert into bagStaple across the bag such that there is space for roots to growAdd 10 to 15 seeds to each bag
3Make a 0.2-g/mL NaCl STOCK solution - Measure 2 grams of salt on balance.Get 10ml of water in a graduated cylinder.Add 2 g of sodium chloride and add water to 10 mL to beaker… this is your stock solution.0.2 g/mL NaClStock Solution
4Decide how many different concentrations you want to do. Don’t forget a control.You will need one seed bag for each concentration.You will need one test tube for each concentration.Get the materials you need.
26Put all the bags upright in the beaker that had the stock solution. Put in dark area for one about one weekDetermine the percent dead for each concentration.Graph with the % dead on the y-axis and concentration on the x-axis.Determine the LD50 or the concentration where 50% of the seeds did not germinate
27Percentage of population killed by a given dose 100755025246810121416Percentage of population killed by a given doseDose (hypothetical units)LD