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© Alere. All rights reserved. Proprietary & confidential. | 1 Performance and Results Interpretation iScreen ® OFD Cotinine Screen
© Alere. All rights reserved. Proprietary & confidential. | 2 iScreen ® OFD Cotinine Screen iScreen OFD Oral Fluid Test Device Rapid on-site screening test device for the detection of cotinine in oral fluid Collect anywhere, anytime Results within minutes Gather all necessary testing supplies iScreen OFD device in foil pouch Timer or stop watch
© Alere. All rights reserved. Proprietary & confidential. | 3 Device Components Device Pouch
© Alere. All rights reserved. Proprietary & confidential. | 4 The Process: Performing the Screen Give Test Device to Donor Note: Test Administrator should not handle the test device once handed to the donor, until the security seal has been applied and Chain of Custody has been established. Instruct donor to open device Have donor remove plastic sponge protector and discard Set aside cranberry device cap Verify that plastic port seal is on cap If not, remove from pouch and insert into cap Port Seal Sponge Protector
© Alere. All rights reserved. Proprietary & confidential. | 5 The Process: Sample Collection Instruct donor on proper procedure for saliva collection Ensure that the donor has not placed anything in mouth for at least 10 minutes prior to collection. Including food, drink, tobacco products, gum, candy, etc. Actively swab inside of mouth and top of tongue for 3 minutes Allow saliva to saturate sponge Sponge should soften completely
© Alere. All rights reserved. Proprietary & confidential. | 6 The Process: Initiating Test Donor secures cranberry cap onto the test device The test device should be held vertically and inserted into the cranberry cap, tightening until the red arrow on the test device is aligned with one of the white marks on the cap. Once the cap is in place, the saliva will wick up the test strips – you will see a pink migration indicating proper saliva flow. If either of the test strips fails to wick, have the donor loosen the cranberry cap ½ to ¾ of a turn and then re- tighten. This allows the air pressure within the device to equalize and the sample to flow properly. Failure to wick properly can be caused by insufficient sample volume, extremely viscous saliva, or an air bubble blocking the flow.
© Alere. All rights reserved. Proprietary & confidential. | 7 The Process: Securing the Device Test Completion Instruct donor to initial and date the silver security seal included in test kit box Donor will place this security seal over device KEEP THE TEST DEVICE IN VIEW OF BOTH THE DONOR AND ADMINISTRATOR UNTIL DEVICE IS SEALED WITH SECURITY SEAL ADMINISTRATOR SHOULD NOT HANDLE DEVICE UNTIL SECURITY SEAL IS IN PLACE
© Alere. All rights reserved. Proprietary & confidential. | 8 Interpret the test results at 10 minutes Results are stable for up to one (1) hour The test strip in the device includes an internal procedural Control (C) ensuring proper device function. Control lines should form next to the C on the test strip. Test interpretation: A line next to the COT or cotinine area a negative test result for that cotinine. The absence of a line next to the COT or cotinine area indicates a presumptive positive result for cotinine. It is recommended that screen results should not be given to donor. Results: Interpretation and Documentation
© Alere. All rights reserved. Proprietary & confidential. | 9 Invalid Test Result The test strip within the device includes an internal procedural Control (C) ensuring proper device function. Control lines should form on the test strip, indicating the test functioned properly. The absence of a Control line on the test strip indicates the test is INVALID. Discard the test device. Perform another test with a new sample and a new device. No Control Line = INVALID Results: Interpretation and Documentation
© Alere. All rights reserved. Proprietary & confidential. | 10 Negative Test Result The appearance of a line next to the COT or cotinine area and the C or control area Discard test device Any indication of a line regardless of color intensity is correctly interpreted as a negative test result. Results: Interpretation and Documentation
© Alere. All rights reserved. Proprietary & confidential. | 11 No line, Presumptive Positive Cotinine Results: Interpretation and Documentation Presumptive Positive Test Result The absence of a line next to the COT or cotinine area indicates a presumptive positive test result for Cotinine. Example shows: presumptive positive test result for Cotinine
© Alere. All rights reserved. Proprietary & confidential. | 12 Thank you. Questions? aleretoxicology.com Technical Services and Questions 800.340.4029
© Alere. All rights reserved. Proprietary & confidential. | 13 Smart. Connected. Empowering.
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