1. CELL-tainer® Single use Bioreactor
November 2013
CellD
20bis, rue du Chapitre
F ROQUEMAURE
Tel : +33 (0)
Fax : +33 (0)

2. CELL-tainer®
A new single-use bioreactor with two-dimensional movement that offers superior mass transfer rates and short mixing times with low shear for high density cell culture and microbial fermentation

3. Features MASS-transfer & mixing
Two dimensional rocking improves mixing and oxygen transfer
Proper and accurate temperature control
Heating by convection (incubator)
Direct cooling (heat exchanger under the bag)
Short mixing times => no gradients
EASY handling & real single use
Bags flexible in volume
Robust design (stackable)
In-line measurements non-invasive,
fully disposable (pH, DO,), bottom mounted,
others (glucose/lactate)
Scaleable
Same process performance at different scales

4. Features Complete system Incubator chamber
Integrated process controls with HMI touchscreen
21 CFR part 11 compliant
PLC controller for rpm, pH, DO, Temp
3 x Gas flow meters
Pressure gauges
2 x Addition pumps
Heavy duty motor
Removable bag tray 4

5. Specifications 5

6. CELL-tainer® single-use bag
Microbial bag 6

7. Benefits
Pre-sterilized and disposable Reduce handling Plug and play operation Quick change over Expansion in a single bag = Minimize contamination risk Reduce variability Save time and labor Cycle time reduction Cost effective

8. 2-dimensional movement

9. kla-value at different rocking speeds
The kla-value determined with the dynamic gassing in/out method including the off-gas analysis.
At the 20 L CELL-tainer, optimal filling volumes are above 8L, rocking speeds above 25 rpm.
At the 200 L CELL-tainer, the optimal filling volume ranges between 90 and 130L. At rocking speeds between 15 and 25 rpm, highest kla-values of 400 h-1 are obtained.

10. Cultivation results 12 L 120 L Cultivation of E. coli (Fed-batch):
Final cell dry mass concentration of 45 gL-1 (OD=140) obtained in 35 h.
(Junne et al, ChemieIngenieurTechnik Vol )

12. Batch and Fed-batch E.coli

13. CELL-tainer® scale up
20L CELL-tainer®
CONFIDENTIAL

14. 200L CELL-tainer®

15. CELL-tainer® 200L single-use bag
Mammalian: 5L – 200L working volume Microbial: 5L – 120L working volume
CONFIDENTIAL 15

16. Service Support 1 Year Manufacturer’s Warranty
Covers mechanical and electronic parts not subject to wear and tear (excluding labor and travel)
Service support
24 hour response with phone/ support to troubleshoot and diagnose problem
If issue is not resolved, an engineer will schedule on-site visit for servicing
Anton Tromper -
Ruben Engelhart -
Lexan Lhu –
Spare parts
Stocked at Charter Medical and available to ship FedEx next day
Optional add-on services / products
Maintenance contract -
Site Acceptance Test (at customer site) - one day for CT20 and 2 days for CT200, excluding travel
Operational training (at customer site) - one day for CT20 and 2 days for CT200, excluding travel
CELL-tainer DACS software for one CELL-tainer including 1 Modbus license
Modbus license for connecting additional CELL-tainer (up to 8)
HMI license for connecting additional CELL-tainer (up to 8)
CONFIDENTIAL

17. THANK YOU For More Information: www.CharterMedical.com
Contact Info:
Dominique Ghozlan
CellD
20bis, rue du Chapitre
F ROQUEMAURE
Tel : +33 (0)
Fax : +33 (0)