Presentation on theme: "CoNISMa, Consorzio Nazionale Interuniversitario Scienze del Mare Partner 1c DISTAM Università degli Studi di Milano Daniele Daffonchio Tullio Brusa Sara."— Presentation transcript:
CoNISMa, Consorzio Nazionale Interuniversitario Scienze del Mare Partner 1c DISTAM Università degli Studi di Milano Daniele Daffonchio Tullio Brusa Sara Borin Enrica Canzi Alessandro Favini Lorenzo Brusetti Claudia Sorlini WP7: screening and characterization of the new products with potential biotechnological application
Metabolic typing of aerobic isolates Screening for: Oil utilisation as unique carbon source Catechol utilization as unique carbon source Esterase activity on long chain satured fatty acids (Tween60) Esterase activity on long chain insatured fatty acids (tryoleine) Production of polysaccarides (acetone precipitation) Antibacterial activity
Metabolic typing of aerobic isolates Strains exhibiting strong activity:
Screening of aerobic isolates for bioconversions Several strains belonging to different phylogenetic groups and isolated from different basins have been tested for biotransformations Isolates have been screened for hydrolysis and/or reduction of a family of test molecules harbouring different substitutions, which are intermediate of drugs biosynthesis
Growing cultures have been inoculated with test molecule and its reduction/hydrolysis products have been extracted and visualized by TLC Screening of aerobic isolates for bioconversions standard Unmodified molecule Modified products
Preliminary screening results: GC, NMR characterisation of bioconversion products Screening for stereoselective reactions
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