2. Introduction to the Microscope  Care, Parts, Focusing  Wet-mount Slides  Biological Drawings

3.  Always carry with 2 hands  Only use lens paper for cleaning  Do not force knobs  Always store covered  Keep objects clear of desk and cords

4. A B J D E F G H L M M L I C K

5. Eyepiece-A B-Body Tube C-Revolving Nosepiece Arm D- Low Objective Lens E- High Objective Lens Stage-F H-Stage Clips Coarse Focus-L Fine Focus- M Base-K G-Diaphragm I, J - Light

6.  Place the Slide on the Microscope  Use Stage Clips  Click Nosepiece to the lowest (shortest) setting  Look into the Eyepiece  Use the Coarse Focus

7. MAGNIFICATION EYE PIECE = 10x LOW POWER = 10x HIGH POWER = 40x TOTAL MAGNIFICATION: Low Power: 10x (eye piece) x 10x (low power objective) = 100x HIGH Power: 10x (eye piece) x 40x (high power objective) = 400x

8.  Follow steps to focus using low power  Click the nosepiece to the longest objective  Do NOT use the Coarse Focusing Knob  Use the Fine Focus Knob to bring the slide What can you find on your slide?

9. Wet-Mount Slides  Materials Needed Compound microscope Slide Cover Slip Dropper Water Specimen

10. HOW TO MAKE A WET MOUNT SLIDE  1. Collect all materials  2. Place sample on slide  3. Place one drop of water onto the specimen  4. Place the cover-slip next to the specimen, holding it at a 45 ° angle, then drop it slowly down atop the water and specimen. **This is to prevent air bubbles!

12. Air Bubble

13. Using your wet-mount slide  Plug the microscope and turn on the lamp.  Before placing the slide under the microscope be sure to Turn the coarse adjustment so the stage is in its lowest position Turn the revolving nosepiece so the lowest power objective is in place

14.  On low power, place the slide under the microscope and bring the specimen into focus by turning the coarse adjustment knob.  While looking through the eyepiece, move the slide to the left, move the slide to the right, move the slide away from you.

15.  Bring the specimen back to the center of the field of view and draw what you see.

16.  Switch the microscope to medium power. Use the fine adjustment know to bring into focus. Use the diaphragm to adjust the amount of light that passes through the specimen.  Draw what you see on Medium power.

17.  Switch to highest power objective, and using the FINE ADJUSTMENT KNOW ONLY, bring specimen into focus.  Draw what you see.

18. Drawing What You See  Now that you’ve viewed your specimen you need to draw it accurately.  The drawing is called a Biological drawing.

19. BIOLOGICAL DRAWINGS THE GOODTHE BAD

20. BIOLOGICAL DRAWING RULES  Use unlined paper.  Draw in pencil.  Always print.  Print the title in capital letters above the field of view.  Drawings and individual structures should be drawn to scale.  When labeling structures on the drawing, do not cross over lines to point to structures.

21. BIOLOGICAL DRAWING RULES  Try not to erase.  Write the name of the specimen below the field of view. When using the scientific name of an organism in places other than the title, the first part of the name is capitalized and the second part of the name is not. Underline the scientific name.  Below the name of the organism and to the right, print your name and the total magnification.

22. BIOLOGICAL DRAWING- Now you try! PENNY One cent (4X objective + 10X eyepiece)

23. MICROSCOPE USE REVIEW 1.CARRY CORRECTLY 2.STAGE ALL THE WAY DOWN 3.START WITH LOW POWER OBJECTIVE 4.USE COARSE FOCUS 5.MOVE TO HIGHER POWER 6.ONLY USE FINE FOCUS OR YOU CAN BREAK THE LENS