1. Light Microscope

2. Eyepiece
Contains a magnifying lens that you look through.

3. Body Tube
Connects the eyepiece to the revolving nosepiece.

4. Revolving Nosepiece
Holds and turns objectives into viewing position.

5. High Power Objective
Contains the lens with the most magnification.

6. Low Power Objective
Contains the lens with the least magnification.

7. Arm
Supports the body tube.

8. Stage
Platform used to support the microscope slide.

9. Stage Clips
Holds the slide in place on the stage.

10. Diaphragm
Regulates the amount of light entering the body tube.

11. Coarse Adjustment
Focuses the image under LOW POWER only!

12. Fine Adjustment
Sharpens the image under high or low magnification. “Fine Tunes” the image!

13. Light Source
Allows light to reflect upward through the diaphragm, the specimen and the lenses.

14. Base
Provides support for the microscope.

15. Steps in Focusing Microscope
Select low power objective and make sure the stage is lowered to its bottom position.
Place the slide on the stage.
Secure the slide with the stage clips.
While looking at the stage, use the coarse adjustment knob to move the stage up to its highest position.
Focus with the COARSE ADJUSTMENT KNOB.

16. Steps in Focusing Microscope
Continue to focus with the FINE ADJUSTMENT KNOB.
After you have the specimen in a clear focus, change to the high power objective.
Focus with the FINE ADJUSTMENT KNOB ONLY!!!
***NEVER EVER EVER EVER USE THE COARSE ADJUSTMENT KNOB WHEN USING THE HIGH POWER OBJECTIVE!!!

17. Steps in Focusing Microscope
When you are done viewing your specimen…
Switch back to the low power objective.
Make sure stage is lowered to its bottom position again.
Remove the slide from the stage.

18. Making A Wet Mount Slide…
Clean the slide and cover slip if needed.
Place a small drop of water in the center of the slide.
Center the specimen in the water.
Place the end of a probe in the water.

19. Making a Wet Mount Slide…
Gently drop the cover slip onto the probe.
Slowly drag the probe through the water and out from under the cover slip.
Check for air bubbles.
Clean the slide and cover slip when you are finished.