3. Always carry with 2 hands Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords

4. Eyepiece Body Tube Revolving Nosepiece Arm Objective Lens Stage Stage Clips Coarse Focus Fine Focus Base Diaphragm Light

5. Follow steps to focus using low power Click the nosepiece to the longest objective Do NOT use the Coarse Focusing Knob Use the Fine Focus Knob to bring the slide What can you find on your slide?

6. Body Tube Nosepiece Objectives Stage Clips Light Ocular lens (Eyepiece) – 10X magnification Arm Stage Coarse Adjustment Fine Adjustment Always carry a microscope with one hand holding the arm and one hand under the base. Base Diaphragm 1. Label the microscope

7. 2-3: What’s my power? To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective. What are the powers of magnification for each of the objectives we have on our microscopes? Fill in the table on your worksheet.

8. Band ColorObjective Power Eyepiece Lens Power Power of Magnification Red Yellow Blue 4x 10x 40x 10x 40x 100x 400x

9. 4. Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

10. Is it best to always use high power? 5. Why can’t you use the largest objective (highest magnification) for some slides? –Sometimes there is not enough room and you will not be able to see it!

11. Place the Slide on the Microscope Use Stage Clips Click Nosepiece to the lowest (shortest) setting Look into the Eyepiece Use low power objective Use the Coarse Focus to focus

12. 6. Try viewing the prepared slides activity...read this slide FIRST then follow directions on the worksheet 1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective into place. 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 4 – When you are done, return the slide you used and continue with the ppt on how to make a wet mount slide. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet!

13. How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of the creek water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. You should NOT use the stage clips when viewing wet-mount slides! – Why? 5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly

14. 8. Make a wet mount slide using the pond water provided. Find an organism or image and draw what you see at three different powers of magnification. Label each drawing. If pond water isn’t available, view a different prepared slide and follow the directions for #8.

15. Practice – these activities can also be found on my webpage under “Scientific Method” Quizlet: U&D Microscope Review for QuizQuizlet: U&D Microscope Review for Quiz PowerPoint Parts of the Microscope Microscope Hangman