1. Introduction to Parasitic Protozoa Not classified under Kingdom Animalia but under Kingdom Protista Main feature is they are unicellular eukaryotes First described some 300 + years ago by Leeuwenhoek when he described Giardia from his own intestine.

2. Classification See Classification beginning on page 49 Subject to much variation Four major types of organisms I will discuss –Flagellated protozoa - Mastigophora –Amoeba - Sarcodina –Ciliated protozoa - Ciliophora –Apicomplexa (sporozoans)

3. Organelles of Locomotion of Mastigophorans Flagella

4. Organelles of Locomotion of Mastigophorans Flagella

5. Gardia lamblia trophozoite

6. Microstructure of Cilium 9 + 2 configuration

7. Sarcodina have Pseudopoda

8. Types of Nutrition Autotrophic = Holophytic or photosynthetic Zooxanthellae which are found in many Cnidaria and other invertebrates are the best examples of these symbiots. Heterotrophic –Holozoic nutrition –Saprozoic nutrition

9. Reproduction in the Protozoa Asexual reproduction –Binary fission –Multiple fission, merogony or schizogony (also sporogony) –Budding

10. Reproduction in the Protozoa Sexual reproduction –Gametogony –Syngamy Isogametes Anisogametes Macrogametes - Microgametes - –Conjugation

11. Trophozoite or Vegetative Stage Entamoeba Giardia lamblia

12. Encystment EntamoebaGiardia lamblia

13. Protozoa Overview Belong to Kingdom Protista Unicellular Eukaryotic Some very important parasites

14. Family Trypanosomatidae Heteroxenous - most members have two phases in the life cycle –first phase, they live in the blood or tissues of some vertebrate (often called hemoflagellates) –second phase is found in the gut of some blood sucking invertebrate (monoxenous) - have a single host

15. Forms found in Trypanosomatidae

16. Trypanosomatidae forms

17. Forms found in Trypanosomatidae

18. Genus Trypanosoma most important flagellates divided into two broad sections based upon development in the invertebrate host –Salivaria - (anterior station development) These accomodate transmission to vertebrate host during the blood meal of the arthropod. –StercorariaPosterior section development. Transmission occurs through fecal contamination

19. Trypanosoma brucei complex Salivaria pleomorphic Vectors are members of the Tsetse fly (genus Glossina Live in the blood, lymph nodes and spleen, and cerebrospinal fluid of the vertebrate host

20. Glossina

21. Trypanosoma brucei complex Live in the blood, lymph nodes and spleen, and cerebrospinal fluid of the vertebrate host Trypanosoma brucei brucei parasite of antelopes and other African ruminants pathogenic to domestic ruminants causes nagana a type of sleeping sickness in animals

22. Trypanosoma brucei gambiense. chronic form of African Sleeping Sickness west central and central Africa vectors include Glossina palpalis and G. tachinoides Game animals are not reservoir hosts.

23. Trypanosoma brucei rhodiense acute form of African Sleeping Sickness east central and central Africa Glossina morsitans, G. pallidipes, and G. swynnertoni are the most important vectors Wild game animals are believed to serve as reservoir hosts.

24. Pathology T. gambiense invades the central nervous system initiating a chronic, sleeping-sickness. apathy, mental dullness, tremor of hands, tongue, etc. followed by convulsions and paralysis. Sleeping follows with coma and death common.

25. Pathology T. rhodiense does not attack the nervous system. causes a more rapid death rapid weight loss, heart involvement, and death can occur within a few months. Winterbottom's sign

26. Diagnosis and Treatment Finding parasites in blood, bone marrow, or cerebrospinal fluid is diagnostic Winterbottom’s sign Today the drug of choice is difluoromethylorithine (DFMO).

27. Epidemiology and Control attempts to control the tsetse fly vectors. Vectors of T. brucie and T. rhodiense occur in open country, pupating in dry, soil vectors of T. gambiense are riverine flies breeding in shady, mosit areas along rivers. Tsetse flies are larviparous Brush removal is effective (febble flyers) but must be maintained.

28. Glossina breeding habitat

29. Cleared Strips to Control Trypanosoma gambiense

30. Epidemiology and Control Chemical spraying with insecticides Elimination of wild game animals. selective breeding of cattle

31. Other Salivarian Trypanosomes T. evansi Causes a disease in horses, camels, elephants, dogs, and other mammals called surra. Nearly 100% fatality to dogs and elephants if untreated can be mechanically transmitted by horseflies T. equiperdum no arthropod host. Veneral transmission, it causes dourine in horses.

32. Stercoraria Trypanosomes Trypanosoma cruzi Chagus discovered the organism in 1910 Infects cone-nosed bugs belonging to the family Reduviidae Causes Chagus disease in humans Distributed throughout Central and South America and infects over 19 million people

33. Vectors of Trypanosoma cruzi

34. Transmission Reduviid bugs usually defecate when they feed Feces contains the infective metacyclic trypanosomes. These enter the host body when the bite is scratched or mucous membranes enter a reticuloendothelial cell of the spleen or liver

35. Transmission Become amasitgote forms and undergo rapid division. Cyst-like pockets of the amastigotes called pseudocyts are formed. Amastigotes complete development and enter the blood where they are infective to the insect vector Infective metacyclid trypomastigotes appear in the feces within 8 - 10 days.

36. Pathogenesis of Chagus Disease Acute phase - most common in children under 5 years Romana's sign Pseudocysts can form in almost any tissue Heart muscle ganglion cells are very susceptible and up to 80% of them may be destroyed. Death may occur within 3-4 weeks after infection.

37. Romana’s sign

38. Pathogenesis of Chagus Disease Chronic stage is most often seen in adults. May last many years damaging heart muscle ganglion cells. In endemic areas, the disease may accont for 70% of the cardiac deaths. megaesophagus or megacolon

39. Pseudocyst of T. cruzi in heart

40. Epidemiology Reduviidae bugs are the most important link to human transmission wild mammals may serve as reservoir hosts, dogs and cats are more important reservoirs for human disease.

41. Epidemiology Transmission can also occur through blood transfusions, contaminated needles, transplacental transmission and ? Breast milk Thatcched roofs and cracked walls are ideal breeding and hiding places for the bugs

42. Diagnosis and Treatment Presence in the blood, cerebrospinal fluid, fixed tissues or lymph Xenodiagnosis is where laboratory reared bugs are allowed to feed on patients and then after a period of time they are examined for flagellates. Complement fixation and other immunodiagnostic tests